L2879

Lysozyme chloride form from chicken egg white

Grade VI, ≥35,000 units/mg protein (E1%/282)

• Synonym(s): Peptidoglycan iV-acetylmuramoyl hydrolase, Mucopeptide N-acetylmuramoylhydrolase, Muramidase
• CAS Number: 9066-59-5
• Enzyme Commission number: 3.2.1.17 (BRENDA, IUBMB)
• EC Number: 232-954-0
• MDL number: MFCD00131557
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: chicken egg white
• Quality Level: 200
• type: Grade VI
• form: lyophilized powder
• specific activity: ≥35,000 units/mg protein (E1%/282)
• mol wt: ~14.3 kDa
• composition: Protein, ≥80%
• technique(s): cell based assay: suitable
• suitability: suitable for cell lysis
• application(s): cell analysis
• storage temp.: −20°C

Description

General description

Lysozyme, also known as muramidase, is a bacteriolytic enzyme found in phagocytic cells like leukocytes. The name "lysozyme" is derived from its ability to dissolve or hydrolyze the β-1-4-glycosidic bond between N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM) in the walls of certain bacteria. This protein is present in animals, plants, bacteria, and viruses. It can also be found in granules of neutrophils, macrophages, as well as in serum, saliva, milk, honey, and hen egg white. There are different types of lysozymes, including the c-type (chicken type), the g-type (goose-type), and the i-type (invertebrates).

Application

Lysozyme chloride form from chicken egg white has been used:

• as a component in the protein mixture to study the effects of yeast cells on membrane fouling caused by a protein mixture in dead-end filtration
• in dip system to study its bacteriostatic or bactericidal effects on Salmonella-contaminated broiler parts
• in viscometric assay for determining lysozyme activity    

Biochem/physiol Actions

Lysozyme serves as an indicator of macrophage-mediated host response, correlates with white cell death, and exhibits a high turnover rate. Elevated levels of serum lysozyme have been observed in various chronic inflammatory conditions, inflammatory bowel diseases, hematological disorders, and renal disorders. The c-type lysozyme from hen egg white is commonly used as a model for studying protein structure and function. Muramidase primarily exhibits bactericidal activity against Gram-positive bacteria.

Unit Definition

One unit will produce a ΔA₄₅₀ of 0.001 per min at pH 6.24 at 25°C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).

Physical form

Lyophilized powder containing sodium chloride and sodium acetate

Preparation Note

3× Crystallized

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves