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L2650

Sigma-Aldrich

Lectin from Glycine max (soybean)

peroxidase conjugate, lyophilized powder

Synonym(s):

SBA, Soybean agglutinin

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

Glycine max (soybean)

conjugate

peroxidase conjugate

Assay

85.00-100.00% (Warburg-Christian)

form

lyophilized powder

potency

<80 μg per mL agglutination activity

peroxidase activity

25-100 units/mg protein

composition

Protein, ~95% modified Warburg-Christian

technique(s)

western blot: suitable

UniProt accession no.

P05046

storage temp.

−20°C

Gene Information

soybean ... LE1(100818710)

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Application

General Western Blot Protocol:
  • Glycoprotein sample size: 500ng
  • Lectin Concentration: 0.1ug/ml

  1. Load samples at 500 ng of glycoprotein per lane
  2. Run 4-20% Bis-Tris SDS page gel
  3. Transfer gel to a PVDF membrane
  4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
  5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
  6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
  7. Detect using chemiluminescent substrate (CPS1-120)

Biochem/physiol Actions

SBA is not blood group specific but has an affinity for N-acetyl-D-galactosamine.

Unit Definition

One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0, at 20 °C.

Physical form

Contains sodium citrate

Preparation Note

Conjugates are prepared from affinity purified lectin.
Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hr incubation at 25 °C.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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C Biel et al.
Archives of toxicology, 96(6), 1815-1827 (2022-04-17)
Human Precision-cut intestinal slices (hPCIS) are used to study intestinal physiology, pathophysiology, drug efficacy, toxicology, kinetics, and metabolism. However, the use of this ex vivo model is restricted to approximately a 24 h timeframe because of declining viability of the hPCIS
A simple method for the preparation of enzyme-antibody conjugates.
M J O'Sullivan et al.
FEBS letters, 95(2), 311-313 (1978-11-15)

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