H8889
Histopaque®-1077 Hybri-Max™
liquid, sterile-filtered, BioReagent, suitable for hybridoma
Synonym(s):
Histopaque 1077
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About This Item
UNSPSC Code:
12352201
NACRES:
NA.75
Recommended Products
sterility
sterile-filtered
product line
BioReagent
form
liquid
technique(s)
cell culture | hybridoma: suitable
single cell analysis: suitable
impurities
endotoxin, tested
density
1.077 g/cm3 at 25 °C (lit.)
storage temp.
2-8°C
General description
Histopaque®-1077 is a solution comprising polysucrose and sodium diatrizoate, adjusted to a density of 1.077 +/- 0.001 g/cm3. This medium facilitates rapid recovery of viable lymphocytes from small volumes of whole blood. This technique is suitable for use in cell-mediated lympholysis and human lymphocyte antigen (HLA)2 typing. It may also be employed as the initial isolation step before the enumeration of T-, B-, and null lymphocytes. It is used to create a density medium for the purification of lymphocytes and other mononuclear cells.
Application
Histopaque®-1077 Hybri-Max™ has been used to isolate:
- peripheral blood mononuclear cells (PBMCs) from heparinized blood by gradient centrifugation
- immune cells (lymphocytes) from peripheral blood
- PBMCs from blood
Legal Information
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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