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Sigma-Aldrich

Glycoprotein Detection Kit

Sufficient for 10 mini-gels (10 × 10 cm) or 5 gels or membranes (16.5 × 19 cm)

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

usage

 kit sufficient for 10 mini-gels (10 × 10 cm)
 kit sufficient for 5 gels or membranes (16.5 × 19 cm)

carbohydrate detection limit measuring range

25-100 ng/band

storage temp.

2-8°C

Application

The Glycoprotein Detection Kit is designed for the selective staining of glycoproteins on polyacrylamide gels and membranes using a modification of the Periodic Acid-Schiff (PAS) method. Staining of sugar moieties of glycoproteins yields magenta bands with a colorless background. The periodic acid/Schiff reagent stains vicinal diol groups found mainly on peripheral sugars and sialic acids and is used as a general glycoprotein stain.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Met. Corr. 1 - Ox. Liq. 1 - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT RE 1 Oral - STOT SE 1

Target Organs

Eyes, Thyroid

Supplementary Hazards

EUH031

Storage Class Code

5.1A - Strongly oxidizing hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sukanya Narasimhan et al.
Nature communications, 8(1), 184-184 (2017-08-05)
The Lyme disease agent, Borrelia burgdorferi, colonizes the gut of the tick Ixodes scapularis, which transmits the pathogen to vertebrate hosts including humans. Here we show that B. burgdorferi colonization increases the expression of several tick gut genes including pixr
Electrophoresis: Theory, Techniques, and Biochemical and Clinical Applications.
Andrews, A.T., ed.
Biochemical and Clinical Applications, 2nd ed, 38-42 (1988)
Silvia Gómez-Sebastián et al.
BMC biotechnology, 12, 59-59 (2012-09-08)
Single-domain antibodies (sdAbs), also known as nanobodies or VHHs, are characterized by high stability and solubility, thus maintaining the affinity and therapeutic value provided by conventional antibodies. Given these properties, VHHs offer a novel alternative to classical antibody approaches. To
Feng Wang-Johanning et al.
Journal of the National Cancer Institute, 104(3), 189-210 (2012-01-17)
The envelope (env) protein of the human endogenous retrovirus type K (HERV-K) family is commonly expressed on the surface of breast cancer cells. We assessed whether HERV-K env is a potential target for antibody-based immunotherapy of breast cancer. We examined
G D Jay et al.
Analytical biochemistry, 185(2), 324-330 (1990-03-01)
Two methods are described for detecting less than 1 microgram of highly glycosylated proteins, such as mucins, on sodium dodecyl sulfate-polyacrylamide gels. They combine commonly employed periodic acid-Schiff (PAS) and Alcian blue dyes with silver stain. Carbohydrate prestaining renders mucins
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