GE17-0440-01

Con-A Sepharose^™ 4B

Cytiva 17-0440-01, pack of 100 mL

• Synonym(s): Concanavalin A Sepharose
• UNSPSC Code: 41106500
• NACRES: NA.56

Properties

• ligand: concanavalin A (con A)
• packaging: pack of 100 mL
• manufacturer/tradename: Cytiva 17-0440-01
• storage condition: (0.1 M acetate buffer pH 6, 1 M NaCl, 1 mM CaCl2, 1 mM MnCl2 and 1 mM MgCl, 20% Ethanol)
• matrix: 4% agarose
• particle size: 45-165 μm
• average diameter: 90 μm
• cleaning in place: 4-9
• working range: 4-6
• storage temp.: 2-8°C

Description

General description

Con A Sepharose^™ 4B with immobilized Concanavalin A is an affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids. Stronger affinity for α-D-glucose than α-D-mannose.

Con A Sepharose^™ is Concanavalin A coupled to Sepharose^™ 4B by the cyanogen bromide method. Concanavalin A (Con A) is a tetrameric metalloprotein isolated from Canavalia ensiformis (jack bean). Con A binds molcules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to Sepharose^™ is routinely used for separation and purification of glycoproteins, polysaccharides and glycolipids.

Features and Benefits

• Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
• Group-specific adsorbent for molecules containing sugars.
• Highly suitable for isolation of cell surface glycoproteins from detergent-solubilized membranes
• Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
• Highly suitable for isolation of cell surface glycoproteins from detergent-solubilized membranes
• Con A coupled to Sepharose^™ 4B via cyanogen bromide activation

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids