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D3937

Sigma-Aldrich

DirectLoad™ 1 kb DNA Ladder

ready-to-use marker for DNA electrophoresis

Synonym(s):

1 kb marker, 1kb gel ladder, 1kb gel marker, 1kb ladder for gel electrophoresis, 1kb marker for gel electrophoresis, DNA ladder, DNA marker, DirectLoad™ marker, RTU DNA ladder, RTU DNA marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker, ready to use DNA ladder, ready to use DNA marker

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About This Item

UNSPSC Code:
41105335
NACRES:
NA.25

Quality Level

200

form

liquid

usage

100 uses

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

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General description

Sigma′s DirectLoad™ 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb. For NA electrophoresis, 5 ul of the marker can be loaded directly into a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting.

Application

Suitable for size determination of dsDNA by DNA electrophoresis with either agarose or polyacrylamide gels.

Features and Benefits

  • Ready-to-load
  • Easy-to-use
  • Popular band sizes

Components

Sigma′s DirectLoad™ 1kb Ladder is provided in a gel-loading solution of 2.5% Ficoll (Type 400), 0.0125% bromophenol blue, and 0.00625% xylene cyanol.

Other Notes

Contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb and 2 kb repeats from 6 to 10 kb.
For optimal resolution, the recommended agarose gel concentration is 0.75%.

Legal Information

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Aron M Geurts et al.
Methods in molecular biology (Clifton, N.J.), 597, 211-225 (2009-12-17)
The genetic dissection of physiological and pathological traits in laboratory model organisms is accelerated by the ability to engineer loss-of-function mutations at investigator-specified loci. This chapter describes the use of zinc-finger nucleases (ZFNs) for the targeted disruption of endogenous rat

Articles

Markers and Ladders for Gel Electrophoresis

We offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments and RNA and can be concurrently run with the samples. All the markers stain well with common nucleic acid stains.

Protocols

End Point PCR Protocol for Long and Accurate DNA Amplification

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.

Antibody-Enzyme Mediated Hot Start PCR Protocol

When using hot start Taq DNA polymerase, the enzyme remains inactive until heated. Hot Start DNA polymerase control is achieved by chemical or antibody modification of the enzyme.

End-Point PCR: Antibody-Mediated Hot Start PCR Protocol with Enhanced Specificity and Yield

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

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