AV3 (HeLa derivative)

88102402, human cervix, Epithelial

• UNSPSC Code: 41106514

Properties

• product name: AV3 (HeLa derivative), 88102402
• biological source: human cervix
• growth mode: Adherent
• karyotype: 2n = 46
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human cervix carcinoma

Cell Line Description

This cell line was originally derived from normal human amnion. The cells are mainly used for virus studies (susceptible to poliovirus 1). HeLa marker chromosomes and type A G6PD have been detected. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line must be considered to be derived from HeLa.

Application

Virus studies: poliovirus 1

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 14
D16S539: 9,10
D5S818: 11,12
D7S820: 12
THO1: 7
TPOX: 8,12
vWA: 16,18

Culture Medium

EMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x100,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Other Notes

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