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biological source
human skin (metastasis)
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
receptors
Not specified
relevant disease(s)
metastasis
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Cell Line Origin
Human melanoma metastatic site lymph node
Cell Line Description
mM466 is a human melanoma derived from a metastatic site (lymph node). Cell Bank Australia reference number CBA-1353.
DNA Profile
Not specified
Culture Medium
RPMI 1640 (R0883) (with 2 mM L-Glutamine (G7513) + 25mM HEPES) + 10% FBS.
Subculture Routine
Optimal split ratio 1:2-1:4 (seeding density 1.4 x 104 cells/cm2). Cells incubated at 37 °C with 5% CO2. Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min. Medium change twice weekly. The cell line MM466 may take up to 72 hours to recover from thaw.
Other Notes
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Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Peter Parsons - Queensland Institute of Medical Research, Australia
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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