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biological source
human lung
growth mode
Adherent
karyotype
Not specified
morphology
Epitheloid/mesenchymal
receptors
Mesothelial-membrane antigen ME1
relevant disease(s)
cancer
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Cell Line Origin
A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure
Cell Line Description
SPC212 was derived from a biphasic tumour of the pleural cavity of a 47-year old female patient, prior to treatment, with a known history of exposure to asbestos.
DNA Profile
STR-PCR Data:
Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 9
D5S818: 11,12
D7S820: 8,10
THO1: 6,9
TPOX: 8
vWA: 16
Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 9
D5S818: 11,12
D7S820: 8,10
THO1: 6,9
TPOX: 8
vWA: 16
Culture Medium
Subculture Routine
Split subconfluent cultures (70-80%) 1:4 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37 °C. Suggested seeding density 1-4 x 10,000 cells/cm2.
Other Notes
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Certificates of Analysis (COA)
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