Vero-Hektor
3092503, African green monkey kidney, Fibroblast-like
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About This Item
UNSPSC Code:
41106514
biological source:
African green monkey kidney
growth mode:
Adherent
karyotype:
Not Specified
morphology:
Fibroblast-like
receptors:
Not specified
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Product Name
Vero-Hektor, 03092503, from African green monkey kidney, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
biological source
African green monkey kidney
growth mode
Adherent
karyotype
Not Specified
morphology
Fibroblast-like
receptors
Not specified
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Cell Line Origin
African green monkey kidney cells, serum-free
Cell Line Description
Vero cell line (Sigma catalog no. 84113001) adapted to grow in the chemically defined protein- and peptide-free medium Hektor G (Cell Culture Technologies). This cell line is substrate dependent, i.e., adherent and may be suitable for the replication of viral particles.
Culture Medium
Hektor G medium (Cell Culture Technologies http://www.cellculture.com/) + 4 mM L-Glutamine (G7513). When freezing cells down use Hektor G medium + 4 mM L-Glutamine (G7513) + 10% DMSO (D2650).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 1-3x10,000 cells/cm2. Use EDTA only (no trypsin); 5% CO2; 37 °C.
Other Notes
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