C4879

α-Chymotrypsinogen A from bovine pancreas

essentially salt-free, lyophilized powder

• Synonym(s): chymotrypsin A zymogen
• CAS Number: 9035-75-0
• EC Number: 232-905-3
• MDL number: MFCD00130483
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: bovine pancreas
• Quality Level: 200
• type: Type II
• form: essentially salt-free, lyophilized powder
• specific activity: ≥40 units/mg solid
• mol wt: 25,656 Da by calculation
• purified by: 6× crystallization
• solubility: 1 mM HCl: soluble 10 mg/mL, clear, colorless
• UniProt accession no.: P00767
• foreign activity: α-chymotrypsin ≤1 U/mg (prior to activation by trypsin)
• storage temp.: −20°C
• Gene Information: cow ... CTRB1(618826)

Description

General description

Chymotrypsinogen from bovine pancreas is a zymogen containing 5 disulfide bridges. It has an isoelectric pH of 8.97.

Application

α-Chymotrypsinogen A from bovine pancreas has been used as model protein crystallization reproducibility studies. It has also been used in the hydrolysis of α-gliadins prior to mass spectroscopy studies.

The enzyme from Sigma has been used in the non-invasive determination of solid-state protein conformation using near infrared (NIR) spectroscopy. It has been used to study the partitioning of protein in polymer/polymer aqueous two-phase systems. The enzyme has also been used for self-interaction chromatography applications, to test the rapid measurement of protein osmotic second virial coefficients. In this technique, the protein is immobilized on chromatographic particles and its retention is measured using isocratic elution.

Biochem/physiol Actions

Chymotrypsinogen A requires limited proteolysis for its activation. Chymotrypsinogen A may be activated by trypsin and chymotrypsin (autolytic activation) to form m α, β, γ, δ and π chymotrypsin (depending upon the conditions of activation). Chymotrypsin is a protease that will preferentially cleave peptides on the carboxyl side of aromatic amino acids including tryptophan, tyrosine, and phenylalanine. It will also hydrolyze peptides on the carboxyl side of leucine, methionine, and alanine.

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met) on the carboxyl end of the peptide bond.

Unit Definition

After activation to Chymotrypsin, one unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

Other Notes

View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves