B3934
Biuret reagent
protein detection level 150-1,000 μg/mL
Synonym(s):
Micro Lowry, Onishi & Barr Modification
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About This Item
form
liquid
protein detection level measuring range
150-1,000 μg/mL
storage temp.
2-8°C
General description
Biuret reagent is used for measuring total protein concentration. It comprises of copper II (cupric ion) alkaline solution, which interacts with the peptide bond to form a blue adduct which is measured at 540nm. Biuret method is less sensitive when compared to other protein estimation methods like Lowry and Folin-Ciocalteu techniques.
Application
Biuret reagent has been used for protein quantification of high-pressure thermal hydrolysis lysate(HPTH), oyster hemolymph and Clostridium cellulovorans (CBDclos) aggregates.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Proteins and Albumin
Roger LB
Laboratory Medicine, 45(1), e25?e41-e25?e41 (2014)
Bacterial inclusion bodies as potential synthetic devices for pathogen recognition and a therapeutic substance release
Talafova K, et al.
Microbial cell factories, 12(1), 16-16 (2013)
Non-lethal determination of sex and reproductive condition of Eastern oysters Crassostrea virginica Gmelin using protein profiles of hemolymph by Proteinchip textregistered and SELDI-TOF-MS technology
Li Y, et al.
Aquaculture (Amsterdam, Netherlands), 309(1-4), 258-264 (2010)
Production of volatile fatty acids by fermentation of waste activated sludge pre-treated in full-scale thermal hydrolysis plants
Morgan-Sagastume F, et al.
Bioresource Technology, 102(3), 3089-3097 (2011)
A simplified method of quantitating protein using the biuret and phenol reagents.
S T Ohnishi et al.
Analytical biochemistry, 86(1), 193-200 (1978-05-01)
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