A9316
Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat
affinity isolated antibody, buffered aqueous glycerol solution
Synonym(s):
Goat Anti-Mouse IgG (whole molecule)−AP
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About This Item
Recommended Products
biological source
goat
conjugate
alkaline phosphatase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous glycerol solution
technique(s)
direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
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General description
Goat polyclonal anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody is reactive with mouse IgG1, IgG2a, IgG2b, IgG3, IgA, and IgM. Cross reactivity of the antibody conjugate is determined by ELISA. The conjugate shows no reactivity with rat IgG or rat serum proteins.
Immunoglobulins (Igs) belong to the immunoglobulin super-family. IgG is an abundant immunoglobulin present in human serum. The four classes of IgG are IgG1, IgG2, IgG3, and IgG4.
Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Application
Anti-Mouse IgG (whole molecule) Alkaline Phosphatase antibody produced in goat has been used as a secondary antibody in:
- histological studies in heart and liver grafts
- immunohistochemical studies of gangliosides and immunostaining of lymphoid organs
- western blot detection in blood samples
Surfactant Protein A was detected in bronchoalveolar fluid using alkaline phosphatase conjugated goat anti-mouse IgG as the secondary at μg/ml in TBS/Tween containing final concentration of 0.5M NaCl.
The level of antibodies specific to P. aeuginosa present in immunized mouse sera was determined by ELISA using alkaline phosphatase-conjugated goat anti-mouse IgG as the secondary antibody at a dilution of 1:1000.
Biochem/physiol Actions
IgG1 deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions and IgG4 is associated with asymptomatic infection.
Other Notes
Antibody adsorbed with rat serum proteins.
Physical form
Solution in 0.05 M Tris, pH 8.0, containing 10 mM glycine, 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide.
Preparation Note
Adsorbed to reduce background with rat samples.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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T Saiki et al.
Transplantation, 71(12), 1806-1815 (2001-07-17)
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Gomez JL, et al.
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Simultaneous immunohistochemical detection of gangliosides and neuronal markers in paraformaldehyde-fixed nervous tissues by acetone etching
Sakumoto Y, et al.
Archives of Histology and Cytology, 72(2), 77-90 (2009)
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Journal of neurology, neurosurgery, and psychiatry, 82(3), 294-299 (2011-01-22)
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Matsuno K, et al.
Archives of Histology and Cytology, 73(1), 1-21 (2010)
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