A3422
Achromopeptidase from bacteria
partially purified powder, ≥20,000 units/mg solid
Synonym(s):
ACP, Achromopeptidase
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About This Item
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
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form
partially purified powder
Quality Level
specific activity
≥20,000 units/mg solid
mol wt
27 kDa
foreign activity
Collagenase, present
storage temp.
−20°C
Application
Achromopeptidase from bacteria may be used:
- in the homogenization step for genomic DNA extraction from microbial biofilm
- in the lysis of gram-positive strains(
- for DNA extraction from Nocardia strains
Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections. Product A3422 is from bacteria and is a partially purified powder containing lactose.
Biochem/physiol Actions
Achromopeptidase is a bacteriolytic enzyme and belongs to serine protease family. It is effective for the lysis of gram positive bacteria compared to lysozyme. Achromopeptidase activity is inhibited in the presence of heavy metals. Achromopeptidase based inhibition assay may be useful for screening the presence of heavy metals.
Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD600 = 0.6 over 2 hours at 37 °C.
Unit Definition
One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.
Physical form
Partially purified powder.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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High Intraspecific Genetic Diversity of Nocardia brasiliensis, a Pathogen Responsible for Cutaneous Nocardiosis Found in France: Phylogenetic Relationships by Using sod and hsp65 Genes
Kosova-Maali D, et al.
BioMed Research International, 2018 (2018)
Achromopeptidase for lysis of anaerobic gram-positive cocci.
Ezaki T and Suzuki S
Journal of Clinical Microbiology, 16(5), 844-846 (1982)
Near real-time inhibitive assay for heavy metals using achromopeptidase
Shukor MY, et al.
Indian Journal of Biotechnology, 13, 398-403 (2014)
Metagenomic analysis of an ecological wastewater treatment plant?s microbial communities and their potential to metabolize pharmaceuticals
Balcom IN, et al.
F1000Research, 5, 844-846 (2016)
Microbial ecosystem analysis in root canal infections refractory to endodontic treatment
Henriques LC, et al.
Journal of Endodontics, 42(8), 1239-1245 (2016)
Protocols
Enzymatic Assay of Achromopeptidase
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