A2404
Alcohol Oxidase solution from Pichia pastoris
buffered aqueous solution, 10-40 units/mg protein (biuret)
Synonym(s):
Alcohol:oxygen oxidoreductase
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
form
buffered aqueous solution
specific activity
10-40 units/mg protein (biuret)
shipped in
dry ice
storage temp.
−20°C
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General description
Alcohol oxidase (AOX) is a homo-octamer, which has a molecular weight of 600-kDa. It has eight flavin adenine dinucleotide (FAD) cofactors. It belongs to the glucose-methanol-choline (GMC) oxidoreductase family.
Application
Alcohol Oxidase may be used to study protein translocation into peroxisomes. Product A2404 is from Pichia pastoris. It has been used for the bacterial expression and immunological verification of Hv-p68 cDNA clones .
Alcohol Oxidase solution from Pichia pastoris has been used in cotton pectin methylesterase (PME) enzyme assay. It has also been used to immobilize onto polyaniline (PANI) film as a visual biosensor in dip-stick format for the determination of ethanol in fermented beverage samples.
Biochem/physiol Actions
Alcohol oxidase catalyzes the oxidation of short-chain, primary, aliphatic alcohols to their respective aldehydes. It has the highest affinity for methanol. Alcohol oxidase is involved in the metabolism of methanol, which is important for yeasts that utilize methanol as their sole energy source. Alcohol oxidase is primarily found in the peroxisome but may be present in the cytoplasm as well.
Unit Definition
One unit will oxidize 1.0 μmole of methanol to formaldehyde per min at pH 7.5 at 25 °C.
Physical form
Solution in 30% sucrose with 0.1 M phosphate buffer at pH 8.0
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Protocols
This procedure may be used for all Alcohol Oxidase products.
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