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Sigma-Aldrich

Leukocyte α-Naphthyl Acetate (Non-Specific Esterase) Kit

Kit formulated with all liquid reagents.

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About This Item

MDL number:
MFCD08705438
UNSPSC Code:
41116123
NACRES:
NA.47

Quality Level

500

form

liquid

shelf life

Expiry date on the label.

IVD

for in vitro diagnostic use

application(s)

hematology
histology

shipped in

wet ice

storage temp.

2-8°C

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Application

Esterases are ubiquitous in nature and encompass a series of different enzymes acting upon select substrates. According to the methodology, α-naphthyl acetate is enzymatically hydrolyzed, liberating a free naphthol compound. This then couples with a diazonium compound, forming black colored deposits at sites of non-specific esterase activity. This enzyme is detected primarily in monocytes, macrophages and histocytes, and is normally absent in granulocytes. Lymphocytes may occasionally exhibit enzyme activity. A sodium fluoride inhibition procedure may be run to demonstrate monocyte inhibition. The procedure is run at 37 °C and may be utilized with blood, bone marrow films, tissue-touch preparations, cytocentrifuge preparations and frozen sections. The enzyme does not survive paraffin processing.

Kit Components Only

Product No.
Description
  • TRIZMAL™ 7.6 Concentrate 50 mL
  • Hematoxylin solution gill no. 3 50 mL

Kit Components Also Available Separately

Product No.
Description
SDS
  • 916α-Naphthyl Acetate Solution 10 mLSDS
  • 915Citrate Solution, pH 3.6±0.1 (25 °C), 27 mM 50 mLSDS
  • 917Fast Blue BB Base Solution 10 mLSDS
  • 914Sodium nitrite solution 10 mLSDS

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - STOT SE 1

Target Organs

Eyes,Central nervous system

Storage Class Code

3 - Flammable liquids

Flash Point(F)

98.1 °F

Flash Point(C)

36.7 °C

Certificates of Analysis (COA)

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