66939
Phalloidin–Atto 425
suitable for fluorescence, ≥90% (HPLC)
Synonym(s):
Atto 425–Phalloidin
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
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Quality Level
Assay
≥90% (HPLC)
manufacturer/tradename
ATTO-TEC GmbH
λ
in methanol
UV absorption
λ: 429-435 nm Amax
suitability
suitable for fluorescence
storage temp.
−20°C
General description
Atto 425 Phalloidin is a novel fluorescent label with a coumarin structure. The dye is intended for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, high fluorescence quantum yield, large Stokes-shift, good photostability, and low molecular weight.Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind F actin in liver and muscle cells. As a result of binding phalloidin, actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin, and not to monomeric actin. The dissociation constant of the actin-phalloidin complex has been determined to be on the order of 3 x 10–8. Phalloidin differs from amanitin in rapidity of action; at high dose levels, death of mice or rats occurs within 1 or 2 hours.
Application
Fluorescent conjugates of phalloidin, rhodamine-phalloidin staining reagents, such as Phalloidin–Atto 425 are used to label actin filaments for histological applications. Some structural features of phalloidin are required for the binding to actin. However, the side chain of amino acid 7 (g-dihydroxyleucine) is accessible for chemical modifications without appreciable loss of affinity for actin.
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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