PPB023
Tris buffer
Pre-set, pH 8.1, pHast Pack™, powder
Synonym(s):
0.1M Tris
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About This Item
UNSPSC Code:
12352200
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General description
Tris buffer, pH 8.1 is a general biological buffer commonly used in biochemistry and molecular biology. It is isotonic and non-toxic in nature due to the presence of tris base. Tris has a buffering capacity range of pH 7.0 - 9.0, with a pKa of 8.1 at 25°C.
Application
Intended for use in:
- Cell culture (sterile filter recommended)
- Western blot
- Protein electrophoresis
- DNA agarose electrophoresis as a component to make Tris acetate-EDTA (TAE) and Tris borate-EDTA (TBE) buffers
Features and Benefits
- Ready-to-use for quick buffer prep
- No measuring and adjusting pH
- Saves time and minimizes effort in the lab
- Biological tests: free of DNase, RNase, protease, and nickase
- Chemical tests: Iron ≤10 ppm, lead ≤5 ppm
Packaging
Foil pouches
Reconstitution
Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 0.1M Tris solution, pH 8.1 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free. This product is suitable for use in cell culture and electrophoresis applications.
Storage and Stability
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Legal Information
pHast Pack is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
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