NIST2372A

Human DNA quantitation standard

NIST^® SRM^® 2372a

• Synonym(s): NIST human DNA quantitation standard
• UNSPSC Code: 41116107
• NACRES: NA.24

Properties

• form: liquid
• packaging: pkg of set (3 x 1 ea)
• manufacturer/tradename: NIST^®
• technique(s): PCR: suitable; qPCR: suitable
• application(s): genomic analysis

Description

General description

This NIST DNA standard comprises three well-characterized human genomic DNA materials in pH 8.0 aqueous buffer. The components are derived from human buffy coat samples and labeled A, B, and C. Their composition is as follows:

• Component A consists of genomic DNA from a single male donor.
• Component B consists of genomic DNA from a single female donor.
• Component C consists of a gravimetric mixture of genomic DNA (1 part male donor : 3 parts female donor).

A unit of the SRM consists of one sterile 0.5 mL vial of each component, each vial containing approximately 55 μL of DNA solution. Each of the vials is labeled and sealed with a color-coded screw cap.

SRM 2372a_cert

SRM 2372a _SDS

Application

Human DNA quantitation standard Standard Reference Material (SRM) is intended for use in the value assignment of human genomic deoxyribonucleic acid (DNA) forensic quantitation materials, primarily those used for quantitative polymerase chain reaction (qPCR).

Features and Benefits

• SRM 2372a available with certificate documenting NIST-certified values of copy number & DNA mass concentration and non-certified values of mitochondrial genomes (mtDNA) relative to nuclear haploid genomes (nDNA)
• Copy number values metrologically traceable to natural units and SI-derived units of volume
• DNA mass concentration values metrologically traceable to natural units and SI-derived units of mass and volume
• Assay-Specific mtDNA/nDNA Ratio values metrologically traceable to droplet digital polymerase chain reaction (ddPCR) measurement
• Expiration details, and storage and use instructions provided on the NIST certificate
• Notes on biomaterial handling, disposal, and transport documented in MSDS

Other Notes

Please download a current certificate at nist.gov/SRM for current analytes and certified values.

Legal Information

NIST is a registered trademark of National Institute of Standards and Technology

SRM is a registered trademark of National Institute of Standards and Technology

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable