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70116

Millipore

Nutrient Agar No 2

suitable for microbiology, NutriSelect® Basic

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About This Item

UNSPSC Code:
41171605
NACRES:
NA.74

Agency

according to ISO 16654:2001
according to ISO 19250:2010
according to ISO 6579-1:2017

Quality Level

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
meat extract, 3 g/L
peptone, 5 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

application(s)

agriculture
bioburden testing
environmental
food and beverages
life science and biopharma

microbiology

suitability

nonselective for Enterococcus spp.
nonselective for Escherichia coli
nonselective for Salmonella spp.
nonselective for Shigella spp.
nonselective for Staphylococcus spp.
nonselective for bacteria (General Media)
nonselective for coliforms

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Application

General purpose medium recommended for sporulation.

Preparation Note

Dissolve 23 g in 1 litre distilled water and adjust the pH to 7.0. Sterilize by autoclaving at 121°C for 15 minutes. For Bacillus strains the addition of 10 mg manganous sulfate monohydrate (Cat. No. 63555) is recommended for sporulation.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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M.O. Odugbo et al.
Journal of Food Protection, 53, 1071-1071 (1990)

Protocols

General guidance for the detection and enumeration of Enterobacteriaceae in food according to EN-ISO 8523:1991 and EN-ISO 4832:1991, respectively.

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