Skip to Content
Merck
All Photos(2)

Key Documents

More Documents

67728

Millipore

C. perfringens Millichrome™ plus Supplement 1

suitable for microbiology

Synonym(s):

C. perfringens chromogenic supplement

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
41171614
NACRES:
NA.74

sterility

sterile; 0.45 μm filtered

Quality Level

200

form

dry powder

shelf life

limited shelf life, expiry date on the label

technique(s)

microbiological culture: suitable

solubility

sterile water: 0.1 g/mL

application(s)

food and beverages
pharmaceutical
water monitoring
microbiology

shipped in

ambient

storage temp.

2-8°C

suitability

selective for bacteria: Clostridium perfringens

General description

C. perfringens Millichrome™ plus Supplement 1 is a selective media supplement for the detection and enumeration of C. perfringens. Millichrome™ chromogenic media is a specialized type of culture medium used to detect and isolate specific groups of bacteria based on their ability to produce enzymes with chromogenic substrates. One vial (10 g) of the supplement is sufficient for 5 L of final media. The prepared supplement is added to the melted C. perfringens Millichrome™ plus Agar Base at 45-50 °C.

Application

C. perfringens Millichrome™ plus Supplement 1 is a selective chromogenic media supplement intended for use in the qualitative detection, differentiation and presumptive identification of C. perfringens from various samples

Packaging

1 vial à 10g (with screw cap) is sufficient for 5L

Preparation Note

  • In a transparent vessel, add 2 g of C. perfringens Millichrome® plus supplement 1 in 20 mL of purified water.
  • Swirl well until complete dissolution.
  • Filter to sterilize at 0.45 μm.
  • Add 20 mL/L of the supplement 1 solution to the melted base at 45/50 °C.

Analysis Note

The Clostridium perfringens is detected by a characteristic orange color after 24 h at an optimal growth temperature of 37°C, while Clostridium difficile forms green-blue to green colonies after incubation at the same temperature

Legal Information

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
MilliChrome is a trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service