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XTGHP-RO

Roche

X-tremeGENE™ HP DNA Transfection Reagent

High-performance polymer reagent for transfecting many cell lines

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About This Item

UNSPSC Code:
41106502
NACRES:
NA.55

grade

for molecular biology

Quality Level

100

form

liquid (aqueous solution)

usage

 mL (suitable for 165 transfections)

packaging

pkg of 0.4 mL (06366244001)
pkg of 1.0 mL (06366236001)
pkg of 5 × 1 mL (06366546001)

manufacturer/tradename

Roche

technique(s)

transfection: suitable

storage temp.

−20°C

General description

X-tremeGENE HP DNA Transfection Reagent is a multi-component reagent that forms a complex with DNA , then transports the complex into animal or insect cells.

Features and Benefits

X-tremeGENE HP DNA Transfection Reagent is a high performance reagent that transfects a broad range of eukaryotic cells, including insect cells and hard-to-transfect cell lines (e.g., Sf9, U-2 OS, MEF, MCF7, Hep G2, PC-3, HeLa, HT-29, HT-1080, K-562, RAW 264.7, Jurkat, PC-12, and HCT 116).

  • Benefit from an easy-to-use non-liposomal reagent that is free of animal-derived components, stable at room temperature, filtered through 0.2 μm pore size membrane, and active in serum-containing medium.
  • Achieve new levels of transfection efficiency in primary cells and tumor cell lines that are not transfected well by other reagents.
  • Generate physiologically relevant data using a reagent with low cytotoxic effects.
  • Increase experimental throughput and enable target evaluation using a simple and consistent protocol.

Quality

Each lot of X-tremeGENE HP DNA Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE HP DNA Transfection Reagent (ratio 3:1 ¼l/¼g DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.

Physical form

Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane. Does not contain any ingredients of human or animal origin.Number of Tests: Using the standard procedure, 1 ml of X-tremeGENE HP DNA Transfection Reagent can be used to perform up to 10,000 transfections in 96-well plates.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

X-tremeGENE is a trademark of Roche

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Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H225 - H319

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

334.4 °F

Flash Point(C)

168 °C

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Glioblastoma constitute the most frequent and deadliest brain tumors of astrocytic origin. They are resistant to all current therapies and are associated with a high rate of recurrence. Glioblastoma were previously shown to respond to treatments by 5-aminolevulinic acid (5-ALA)-based
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