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UFC5100

Millipore

Amicon® Ultra Centrifugal Filter, 100 kDa MWCO

sample volume 0.5 mL, regenerated cellulose membrane, MWCO 100 kDa

Synonym(s):

Centrifugal concentrator, 100 kDa MWCO, 0.5 mL sample volume

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About This Item

UNSPSC Code:
41104916
eCl@ss:
36100101
NACRES:
NB.24

material

polypropylene cap
polypropylene tube
regenerated cellulose membrane
styrene-butadiene housing

Quality Level

400

sterility

non-sterile

product line

Amicon®

manufacturer/tradename

Amicon® Ultra

parameter

0.5 mL sample volume

technique(s)

protein extraction: suitable
protein purification: suitable

L

4.99 cm (1.96 in.)

diam.

10.8 mm

filtration area

1 cm2

volume

0.5 mL

pore size

100 kDa MWCO

shipped in

ambient

General description

Amicon® Ultra-0.5 centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, IgG (156 kDa) at 1 mg/ml initial concentration is typically retained at >95% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.

Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.

Application

  • Ultrafiltration with 100,000 Dalton molecular weight cutoff Ultracel® membrane
  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
  • Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC
  • Desalting, buffer exchange, and protein dialysis

Features and Benefits

  • Ultracel® regenerated cellulose membrane with 100 kDa NMWCO delivers retentate recovery of greater than 90%
  • Concentrates 500 μL down to 15 μL
  • Concentration factor 25x to 30x
  • High sample recovery (greater than 90% of dilute starting solution)
  • Fast processing time: 10 to 30 minutes
  • Reverse spin capability provides consistent recoveries

Linkage

Replaces: 42414

Physical form

Color Code: Clear

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10-13 - German Storage Class 10 to 13

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