All Photos(3)

Documents

COA/COQ

MABF267

Anti-Cochlin Antibody/COCH, clone 9A10D2

Name: Anti-Cochlin Antibody/COCH, clone 9A10D2
Brand: MM

clone 9A10D2, from rat

Synonym(s):

Cochlin, COCH-5B2

Sign Into View Organizational & Contract Pricing

All Photos(3)

About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

biological source

rat

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9A10D2, monoclonal

species reactivity

human, mouse

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_001128530

UniProt accession no.

O43405

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... COCH(1690)

General description

The protein Cochlin/COCH is important in the development of the cochlea and vestibule in mammals as well as other functions. Cochlin/COCH is an extracellular matrix protein that plays a role in cell shape, positioning and motility. Recent research shows that it is also important in our innate immune response to bacteria and pathogens. Upon bacterial infection, a fragment of Cochlin/COCH is released from the conduits of B cell follicles in the spleen and this triggers protective cytokines in the periphery. Cochlin/COCH is expressed in epithelial tissues in various tissues, particularly in follicular cells and network of conduits in the spleen and lymph nodes. Cochlin/COCH also is a target for BMP4 mediation in embryonic stem cells (ESCs) where it facilitates self-renewal. The protein also appears to suppress neural differentiation of ESCs and suggests that Cochlin/COCH protein is important in ES cell fate determination. Mutations in the Cochlin/COCH gene are responsible for human DFNA9 syndrome, a disorder characterized by neurodegeneration of the inner ear that leads to hearing loss and vestibular impairments.

Immunogen

Recombinant protein corresponding to human Cochlin/COCH.

Application

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This Anti-Cochlin Antibody/COCH, clone 9A10D2 is validated for use in Western Blotting and Immunohistochemistry for the detection of Cochlin/COCH.

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Cochlin/COCH in 10 µg of human lymph node tissue lysate.
Western Blotting Analysis: A representative lot detected Cochlin/COCH in spleen and lymph node from WT and COCH knockout mice (Py, B.F., et al. (2013). Immunity. 38:1063-1072).
Western Blotting Analysis: A representative lot detected Cochlin/COCH in protein cleaved into p18 and p8 products. (Py, B.F., et al. (2013). Immunity. 38:1063-1072).
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Cochlin/COCH in mouse spleen tissue.
Immunohistochemistry Analysis: A representative lot detected Cochlin/COCH in spleen and lymphnode from WT and Coch knockout mice (Py, B.F., et al. (2013). Immunity. 38:1063-1072).

Quality

Evaluated by Western Blotting in mouse spleen tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Cochlin/COCH in 10 µg of mouse spleen tissue lysate.

Target description

~59 kDa observed. This protein is cleaved into p18 and p8 products. Uncharacterized bands may be observed in some lysates.

Physical form

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Cochlin Deficiency Protects Aged Mice from Noise-Induced Hearing Loss.

Dorien Verdoodt et al.

International journal of molecular sciences, 22(21) (2021-11-14)

Several studies have shown that type IV fibrocytes, located in the spiral ligament, degenerate first after noise exposure. Interestingly, this is the region where Coch expression is most abundant. As it is suggested that cochlin plays a role in our

Cochlin produced by follicular dendritic cells promotes antibacterial innate immunity.

Py, BF; Gonzalez, SF; Long, K; Kim, MS; Kim, YA; Zhu, H; Yao, J; Degauque, N; Villet et al.

Immunity null

Different Phenotypes of the Two Chinese Probands with the Same c.889G>A (p.C162Y) Mutation in COCH Gene Verify Different Mechanisms Underlying Autosomal Dominant Nonsyndromic Deafness 9.

Qi Wang et al.

PloS one, 12(1), e0170011-e0170011 (2017-01-19)

By analyzing the different phenotypes of two Chinese DFNA9 families with the same mutation located in the intervening region between the LCCL and vWFA domains of cochlin and testing the functional changes in the mutant cochlin, we investigated the different

Roquin paralogs 1 and 2 redundantly repress the Icos and Ox40 costimulator mRNAs and control follicular helper T cell differentiation.

Vogel, KU; Edelmann, SL; Jeltsch, KM; Bertossi, A; Heger, K; Heinz, GA; Zoller, J; Warth et al.