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MABF2250

Sigma-Aldrich

Anti-NKp44 (CD336) Antibody, clone 3.43.13

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

antibody form

purified antibody

clone

3.43.13
monoclonal

purified by

using protein G

species reactivity

mouse, human, human

concentration

(Please refer to lot specific datasheet.)

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable

isotype

IgG1κ

UniProt accession no.

General description

NKp44-human crystallizable fragment (Fc) fusion protein.

Specificity

Clone 3.43.13 is a mouse monoclonal antibody that detects human Natural cytotoxicity triggering receptor 2 (NKp44) protein.

Application

Affects Function Analysis: A representative lot activated both NK92-44wt and NK92-44mut cell lines (Brusilovsky, M., et. al. (2015). Eur J Immunol. 45(4):1180-91).


Immunocytochemistry Analysis: A representative lot detected NKp44 (CD336) in Immunocytochemistry applications (Brusilovsky, M., et. al. (2015). Eur J Immunol. 45(4):1180-91).

Flow Cytometry Analysis: A representative lot detected NKp44 (CD336) in Flow Cytometry applications Barrow, A.D., et. al. (2018). Cell. 172(3):534-548; Kikuchi-Maki, A., et. al. (2003). J Immunol. 171(7):3415-25; Fuchs, A., et. al. (2005). Blood. 106(6):2076-82; Brando, C., et. al. (2005). J Leukoc Biol. 78(2):359-71; Eisenberg, V., et. al. (2017). Front Immunol. 8:1212).

Quality

Evaluated by Flow Cytometry in rhIL-2 stimulated human peripheral blood mononuclear cells (PBMCs) for 7 days (100 U per mL).

Flow Cytometry Analysis: 1 µg of this antibody detected NKp44 (CD336) in rhIL-2 stimulated one million human peripheral blood mononuclear cells (PBMCs) for 7 days (100 U per mL).

Physical properties

30.68 kDa calculated.

Physical form

Purified mouse monoclonal antibody IgG1 in PBS without any preservatives.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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