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MABE986

Sigma-Aldrich

Anti-PTB Antibody, clone BB7

clone Bb7, from mouse

Synonym(s):

Polypyrimidine tract-binding protein 1, PTB, 57 kDa RNA-binding protein PPTB-1, Heterogeneous nuclear ribonucleoprotein I, hnRNP I, PTB

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

Bb7, monoclonal

species reactivity

mouse, human

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

NP_002810

UniProt accession no.

P26599

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PTBP1(5725)

General description

Polypyrimidine tract-binding protein 1 (UniProt P26599; also known as 57 kDa RNA-binding protein PPTB-1, Heterogeneous nuclear ribonucleoprotein I, Heterogeneous nuclear ribonucleoprotein polypeptide I, hnRNP I, PTB, RNA-binding protein) is encoded by the PTBP1 (also known as HNRNP-I, HNRNPI, HNRPI, PTB, PTB2, PTB3, PTB4, PTB-1, PTB-T) gene (Gene ID 5725) in human. The serine–arginine rich (SR) proteins and the heterogeneous nuclear ribonucleoproteins (hnRNPs) are the most typical families of RBPs involved in splicing regulation. SR proteins generally play a positive role in exon recognition by binding exonic and intronic enhancers near the alternative splice sites. On the other hand, hnRNPs often bind to silencer elements and interfere with the recruitment of spliceosome components or positive regulators. PTB, also known as hnRNP I, belongs to an hnRNP family of RNA-binding proteins and is involved in several aspects of cellular mRNA metabolism including splicing, RNA stability, and internal ribosome entry site (IRES)-mediated translation of viral and cellular mRNAs. PTB functions as a splicing repressor by preventing recruitment of U2AF65 or correct base pairing of U2 snRNP, or by affecting the interaction of U1 snRNP with other components of the spliceosome.

Immunogen

Recombinant protein human PTB.

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
This Anti-PTB Antibody, clone BB7 is validated for use in Immunocytochemistry, Western Blotting and Immunoprecipitation for the detection of PTB .
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected PTB in 10 µg of HT-29 and HEK293 cell lysate.
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected PTB in HeLa, HUVEC, and NIH/3T3 cells.
Immunoprecipitation Analysis: A representative lot immunoprecipitated PTB in HeLa and WERI-1 nuclear extracts. (Chou, M.Y., et al. (2000). Mol Cell. 5(6):949-57).
Western Blotting Analysis: A representative lot detected PTB in HeLa and WERI-1 nuclear extracts (Sharma, S., et al. (2005). Mol Cell. 19(4):485-496).

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected PTB in 10 µg of HeLa cell lysate.

Target description

~57 kDa observed. The target band may appear as a doublet, most likely due to the presence of spliced isoforms. Isoform 1 (57.2 kDa calculated), isoform 2/PTB2 (59.0 kDa calculated), isoforms 3 (59.6 kDa calculated).

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Multisite RNA binding and release of polypyrimidine tract binding protein during the regulation of c-src neural-specific splicing.
Chou, MY; Underwood, JG; Nikolic, J; Luu, MH; Black, DL
Molecular Cell null
Developmental control of CaV1.2 L-type calcium channel splicing by Fox proteins.
Tang, ZZ; Zheng, S; Nikolic, J; Black, DL
Molecular and cellular biology null
Polypyrimidine tract binding protein blocks the 5' splice site-dependent assembly of U2AF and the prespliceosomal E complex.
Sharma, S; Falick, AM; Black, DL
Molecular Cell null
An inducible change in Fox-1/A2BP1 splicing modulates the alternative splicing of downstream neuronal target exons.
Lee, JA; Tang, ZZ; Black, DL
Genes & Development null
Lauren T Gehman et al.
Nature genetics, 43(7), 706-711 (2011-05-31)
The Rbfox family of RNA binding proteins regulates alternative splicing of many important neuronal transcripts, but its role in neuronal physiology is not clear. We show here that central nervous system-specific deletion of the gene encoding Rbfox1 results in heightened

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