MAB360
Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5
ascites fluid, clone GA5, Chemicon®
Synonym(s):
GFAP
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
GA5, monoclonal
species reactivity
rat, human, mouse, rabbit, chicken, pig, bovine
packaging
antibody small pack of 25 μL
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
ambient
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... GFAP(2670)
Related Categories
General description
Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
Specificity
Glial fibrillar acidic protein. On western blots of extracts from a human glioma cell line (U33CG/343MG), MAB360 recognizes a band at approximately 51 kDa corresponding to GFAP (Debus, 1983). By immunohistochemistry it recognizes astrocytes and Bergmann glia cells, glioma and glial cell derived tumors. Shows no cross-reactivity with vimentin.
Reactivity with other species has not been determined.
Immunogen
Purified GFAP from porcine spinal cord.
Application
Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 detects level of Glial Fibrillary Acidic Protein & has been published & validated for use in IC, IH, IH(P) & WB with more than 65 product citations.
Immunocytochemistry:
A previous lot was used on cultured mammalian cells.
Immunohistochemistry(paraffin):
1:400-1:800 dilution from a previous lot was used on alcohol-fixed paraffin embedded sections of rat brain (cerebrum or cerebellum) and human brain.
Immunoblotting: A 1:1000 dilution of a previous lot was used.
Optimal working dilutions must be determined by the end user
A previous lot was used on cultured mammalian cells.
Immunohistochemistry(paraffin):
1:400-1:800 dilution from a previous lot was used on alcohol-fixed paraffin embedded sections of rat brain (cerebrum or cerebellum) and human brain.
Immunoblotting: A 1:1000 dilution of a previous lot was used.
Optimal working dilutions must be determined by the end user
Quality
Routinely evaluated by Western Blot on Mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected GFAP on 10 μg of Mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected GFAP on 10 μg of Mouse brain lysates.
Target description
~ 51 kDa
Linkage
Replaces: 04-1031; 04-1062
Physical form
Unpurified mouse monoclonal IgG1 liquid in buffer containing 15 mM sodium azide.
Analysis Note
Control
Positive Control: Cultured astrocytes, brain tissue (white matter), spinal chord, retina
Positive Control: Cultured astrocytes, brain tissue (white matter), spinal chord, retina
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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O Nesic et al.
Neuroscience, 143(3), 779-792 (2006-11-01)
The effect of spinal cord injury (SCI) on the expression levels and distribution of water channel aquaporin 4 (AQP4) has not been studied. We have found AQP4 in gray and white matter astrocytes in both uninjured and injured rat spinal
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Das, M; Patil, S; Bhargava, N; Kang, JF; Riedel, LM; Seal, S; Hickman, JJ
Biomaterials null
Expression of leptin and leptin receptor isoforms in the rat and human carotid body.
Porzionato A, Rucinski M, Macchi V, Stecco C, Castagliuolo I, Malendowicz LK, De Caro R
Brain Research null
Cellular organization of adult neurogenesis in the Common Marmoset.
Bunk, Eva C, et al.
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