MAB3118
Anti-DYKDDDDK (FLAG® epitope tag) Antibody, clone 2EL-1B11
ascites fluid, clone 2EL-1B11, Chemicon®
Synonym(s):
Anti-FLAG Antibody, FLAG Tag Antibody
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43
Recommended Products
biological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
2EL-1B11, monoclonal
species reactivity
vertebrates, E. coli
species reactivity (predicted by homology)
rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
shipped in
dry ice
target post-translational modification
unmodified
General description
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
FLAG® is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
FLAG® is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
Specificity
DYKDDDDK sequence containing proteins. Reacts with both N-terminal and C-terminal fusion proteins.
Immunogen
Epitope: Epitope tag
Synthetic peptide M-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys(C) coupled to ovalbumin
Application
Detect DYKDDDDK (FLAGepitope tag) using this Anti-DYKDDDDK (FLAG epitope tag) Antibody, clone 2EL-1B11 validated for use in ELISA, IC, IF, IP & WB.
Immunofluorescence:
A 1:1,000-1:5,000 dilution of a previous lot was used in IF.
Immunoprecipitation:
A 1:1,000-1:5,000 dilution of a previous lot was used IP on recombinant proteins.
ELISA:
A 1:1,000-1:5,000 dilution of a previous lot was used in Enzyme-linked immunosorbent assay on recombinant proteins.
Note: Does not require Ca++ for binding.
Immunoblotting:
on recombinant proteins
Optimal working dilutions must be determined by the end user.
A 1:1,000-1:5,000 dilution of a previous lot was used in IF.
Immunoprecipitation:
A 1:1,000-1:5,000 dilution of a previous lot was used IP on recombinant proteins.
ELISA:
A 1:1,000-1:5,000 dilution of a previous lot was used in Enzyme-linked immunosorbent assay on recombinant proteins.
Note: Does not require Ca++ for binding.
Immunoblotting:
on recombinant proteins
Optimal working dilutions must be determined by the end user.
Research Category
Epitope Tags & General Use
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Epitope Tags
Quality
Routinely evaluated by Western Blot on Flag-mTor protein blot.
Western Blot Analysis:
1:500 dilution of this lot detected Flag mTor on Flag-mTOR blot.
Western Blot Analysis:
1:500 dilution of this lot detected Flag mTor on Flag-mTOR blot.
Linkage
Replaces: AB3250
Physical form
Ascites mouse monoclonal IgG1 liquid containing no preservative.
Unpurified
Storage and Stability
Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Cells transfected with FLAG-tagged fusion vector
Cells transfected with FLAG-tagged fusion vector
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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