MAB3086
Anti-Ryanodine Receptor Antibody, clone RYR.1
clone RYR.1, Chemicon®, from mouse
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
RYR.1, monoclonal
species reactivity
rat, human, mouse, pig, bovine, canine
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable
isotype
IgG2b
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... RYR1(6261)
Specificity
Reacts with the C-terminus cytoplasmic domain of ryanodine receptor. Ryanodine receptors have been shown to play critical roles in the intracellular Ca2+ signaling occurring during cell activation in muscle cells and non-muscle cells. MAB3086 reacts with ryanodine receptor (MW approx. 500kDa) isolated from a variety of cell types (e.g. lymphocytes, macrophages, granulocytes, fibroblasts, epithelial, endothelial cells, skeletal muscle, cardiac muscle and brain tissues).
Immunogen
Short synthetic polypeptide corresponding to the c-terminal domain of the ryanodine receptor.
Application
Anti-Ryanodine Receptor Antibody, clone RYR.1 detects level of Ryanodine Receptor & has been published & validated for use in ELISA, FC, IC, IH, IP, RIA & WB.
Immunoblot: 1:100 using enhanced chemiluminescence detection. It is suggested that you perform a dot blot to verify presence of the RyR protein if you are having difficulties performing an immunoblot assay.
Immunocytochemistry: 1:100 by indirect immunofluorescence. Visualization by confocal microscopy is required, as detection by standard fluorescent microscopy will not be adequate to detect the RyR. Additionally, fluorescent, not enzymatic, detection is required. Due to the intensity of confocal lasers, use of an anti-fading agent, such as DABCO, is strongly recommended.
Immunoprecipitation: 1:100
Flow cytometry: 1:500
ELISA: 1:1000
Immunohistochemistry: 1:100
RIA: 1:5000
Optimal working dilutions must be determined by the end user.
Immunocytochemistry: 1:100 by indirect immunofluorescence. Visualization by confocal microscopy is required, as detection by standard fluorescent microscopy will not be adequate to detect the RyR. Additionally, fluorescent, not enzymatic, detection is required. Due to the intensity of confocal lasers, use of an anti-fading agent, such as DABCO, is strongly recommended.
Immunoprecipitation: 1:100
Flow cytometry: 1:500
ELISA: 1:1000
Immunohistochemistry: 1:100
RIA: 1:5000
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Signaling Neuroscience
Ion Channels & Transporters
Signaling Neuroscience
Physical form
Format: Purified
Purified immunoglobulin from culture supernatant. In 10 mM sodium phosphate, 150 mM NaCl, 0.01% NaN3, pH. 7.5
Storage and Stability
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Ryanodine receptor-ankyrin interaction regulates internal Ca2+ release in mouse T-lymphoma cells.
Bourguignon, L Y, et al.
The Journal of Biological Chemistry, 270, 17917-17922 (1995)
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