MAB1273
Anti-Mitochondria Antibody, surface of intact mitochondria, clone 113-1
clone 113-1, Chemicon®, from mouse
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
113-1, monoclonal
species reactivity
human
should not react with
mouse, rat
manufacturer/tradename
Chemicon®
technique(s)
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
input
sample type neural stem cell(s)
isotype
IgG1
shipped in
dry ice
target post-translational modification
unmodified
General description
This antibody recognizes a 60 kDa nonglycosylated protein component of mitochondria found in human cells. It can be used to stain mitochondria in cell, tissue or biochemical preparations and can be used as a marker of the mitochondria in subcellular fractions.
Specificity
Gives mitochondrial staining on all human cell types and does not cross react with rat and mouse tissue. The staining pattern is best described as a spaghetti-like staining pattern in fibroblasts and a perinuclear large speckled pattern in lymphonoid cells.
MAB1273 recognizes a 65 kD protein by immunoprecipitation. Antibody produces the stringy, "spaghetti-like" staining patten in the cytoplasm of human cells.
MAB1273 recognizes a 65 kD protein by immunoprecipitation. Antibody produces the stringy, "spaghetti-like" staining patten in the cytoplasm of human cells.
Reactivity with other species has not been determined
Immunogen
Epitope: surface of intact mitochondria
Application
Anti-Mitochondria Antibody, surface of intact mitochondria, clone 113-1 is an antibody against Mitochondria for use in IF, IH, IH(P) & IP.
Immunohistochemistry:
1:100 dilutoin from a previous lot was used. Works best with organic fixatives (acetone, methanol, etc). 2% PFA for 10-15 min can be used but stronger use of formalin or 4% PFA limits staining.
The antibody has been used to isolate intact mitochondria from freeze/thaw lysates of living cells via conjugation to magnetic beads {http://www.dynalbiotech.com}.
Immunohistochemistry in paraffin embedded tissues:
Requires citric acid/microwave antigen retrieval; enhanced ABC or amplified detection systems recommended.
Optimal Staining With EDTA Buffer, pH 8.0, Epitope Retrieval: Heart Ventricle Epithelium
The antibody has been used to isolate intact mitochondria from freeze/thaw lysates of living cells via conjugation to magnetic beads {http://www.dynalbiotech.com}.
Indirect immunofluorescence:
1:10-1:50 dilution from a previous lot was used, 30 - 100 μL per slide well.
MAB1273 recognizes a 65 kD protein by immunoprecipitation.
Optimal working dilutions and protocols must be determined by the end user.
1:100 dilutoin from a previous lot was used. Works best with organic fixatives (acetone, methanol, etc). 2% PFA for 10-15 min can be used but stronger use of formalin or 4% PFA limits staining.
The antibody has been used to isolate intact mitochondria from freeze/thaw lysates of living cells via conjugation to magnetic beads {http://www.dynalbiotech.com}.
Immunohistochemistry in paraffin embedded tissues:
Requires citric acid/microwave antigen retrieval; enhanced ABC or amplified detection systems recommended.
Optimal Staining With EDTA Buffer, pH 8.0, Epitope Retrieval: Heart Ventricle Epithelium
The antibody has been used to isolate intact mitochondria from freeze/thaw lysates of living cells via conjugation to magnetic beads {http://www.dynalbiotech.com}.
Indirect immunofluorescence:
1:10-1:50 dilution from a previous lot was used, 30 - 100 μL per slide well.
MAB1273 recognizes a 65 kD protein by immunoprecipitation.
Optimal working dilutions and protocols must be determined by the end user.
Quality
Routinely evaluated by immunohistochemistry on heart ventricle cells.
Immunohistochemistry(paraffin) Analysis:
Mitochondria (cat. # MAB1273) staining on heart ventricle cells. Tissue pretreated with EDTA, pH 8.0This lot of antibody was diluted to 1:80, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seeing the circular-granular layer surrounding the nucleus of the cells.
Optimal Staining With EDTA Buffer, pH 8.0, Epitope Retrieval: Heart Ventricle Epithelium
Immunohistochemistry(paraffin) Analysis:
Mitochondria (cat. # MAB1273) staining on heart ventricle cells. Tissue pretreated with EDTA, pH 8.0This lot of antibody was diluted to 1:80, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seeing the circular-granular layer surrounding the nucleus of the cells.
Optimal Staining With EDTA Buffer, pH 8.0, Epitope Retrieval: Heart Ventricle Epithelium
Target description
65 kDa
Physical form
Format: Purified
Purified mouse monoclonal IgG1 in buffer containing liquid in PBS. Contains no preservative. Concentrated hybridoma supernatant.
Storage and Stability
Stable for 6 months at -20°C in undiluted aliquots from date of receipt.
Analysis Note
Control
COS7 cells.
COS7 cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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C Herrnstadt et al.
Genomics, 60(1), 67-77 (1999-08-25)
We describe here a nuclear mitochondrial DNA-like sequence (numtDNA) that is nearly identical in sequence to a continuous 5842 bp segment of human mitochondrial DNA (mtDNA) that spans nucleotide positions 3914 to 9755. On the basis of evolutionary divergence among
Selective loss of KGDHC-enriched neurons in Alzheimer temporal cortex: does mitochondrial variation contribute to selective vulnerability?
L W Ko, K F Sheu, H T Thaler, W R Markesbery, J P Blass
Journal of Molecular Neuroscience null
EGR2 induces apoptosis in various cancer cell lines by direct transactivation of BNIP3L and BAK.
Unoki, M; Nakamura, Y
Oncogene null
J P Jaffrézou et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 15(1), 123-133 (2001-01-10)
The subcellular origin of ceramide signaling in ionizing radiation-triggered apoptosis was investigated using two previously described subclones of the autonomous erythro-myeloblastic cell line TF-1, radio-resistant and -sensitive TF-1-34 and TF-1-33, respectively. We show in nuclei-free lysates and cytoplasts that both
L Abu-Elheiga et al.
Proceedings of the National Academy of Sciences of the United States of America, 97(4), 1444-1449 (2000-03-04)
Animals, including humans, express two isoforms of acetyl-CoA carboxylase (EC ), ACC1 (M(r) = 265 kDa) and ACC2 (M(r) = 280 kDa). The predicted amino acid sequence of ACC2 contains an additional 136 aa relative to ACC1, 114 of which
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