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CC43

Sigma-Aldrich

Anti-Cdh1 (Ab-2) Mouse mAb (DH01)

liquid, clone DH01, Calbiochem®

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

DH01, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... CDH1(999)

General description

Anti-Cdh1 (Ab-2), mouse monoclonal, clone DH01, recognizes the ~55 kDa Cdh-1 protein in Rat-1 cells. It is validated for Western blotting and immunoprecipitation.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the ~55 kDA Cdh1 protein.
Recognizes the ~55 kDa Cdh-1 protein in Rat-1 cells.

Immunogen

Human
recombinant human Cdh1 protein

Application


Immunoblotting (1 g/ml)
Immunoprecipitation (2 g/mg protein lysate)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 10 mM PBS, 0.2% BSA, pH 7.4.

Analysis Note

Negative Control
Normal mouse serum
Positive Control
Junkat or LS174T cells

Other Notes

Does not cross-react with other WD repeat containing proteins, including Cdc20. Antibody should be titrated for optimal results in individual systems.
Kramer, E.R., et al. 2000 Mol. Biol. Cell11, 1555.
Petersen, B.O., et al. 2000 Genes Dev.14, 2330.
Pfleger, C.M., et al. 2001 Genes Dev.15, 1759.
Sorensen, C.S., et al. 2001 Mol. Cell Biol.21, 3692.
Gieffers, C., et al. 1999 Proc. Natl. Acad. Sci. USA96, 11317.
Visintin, R., et al. 1997 Science278, 460.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cell reports, 1(5), 434-443 (2012-06-19)
The NFkB/Rel family of proteins play critical roles in a variety of cellular processes. Thus, their physiological activation is tightly controlled. Recently, the NFkB2/p100 precursor has been characterized as the fourth IkB type of suppressor for NFkB. However, the molecular
Dipankar Ray et al.
Molecular and cellular biology, 25(8), 3338-3347 (2005-03-31)
Ubiquitin-dependent degradation of Cdc25A is a major mechanism for damage-induced S-phase checkpoint. Two ubiquitin ligases, the Skp1-cullin-beta-TrCP (SCFbeta-TrCP) complex and the anaphase-promoting complex (APCCdh1), are involved in Cdc25A degradation. Here we demonstrate that the transforming growth factor beta (TGF-beta)-Smad3 pathway
Jia Liu et al.
Cell discovery, 2, 15044-15044 (2016-07-28)
Anaphase-promoting complex/cyclosome/Cdh1 is a multi-subunit ubiquitin E3 ligase that drives M to G1 cell cycle progression through primarily earmarking various substrates for ubiquitination and subsequent degradation by the 26S proteasome. Notably, emerging evidence suggested that Cdh1 could also function in
Kyung Uk Hong et al.
The Journal of biological chemistry, 284(24), 16501-16512 (2009-04-17)
During mitosis, establishment of structurally and functionally sound bipolar spindles is necessary for maintaining the fidelity of chromosome segregation. Tumor-associated microtubule-associated protein (TMAP), also known as cytoskeleton-associated protein 2 (CKAP2), is a mitotic spindle-associated protein whose level is frequently up-regulated
Savvas C Pavlides et al.
Cell cycle (Georgetown, Tex.), 15(7), 931-947 (2016-03-11)
We previously reported that aberrant TGF-β/Smad2/3 signaling in endometrial cancer (ECA) leads to continuous ubiquitylation of p27(kip1)(p27) by the E3 ligase SCF-Skp2/Cks1 causing its degradation, as a putative mechanism involved in the pathogenesis of this cancer. In contrast, normal intact
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