116816

Adenosine 3′,5′-cyclic Monophosphorothioate, 8-Bromo-, Rp-Isomer, Sodium Salt

A potent, cell-permeable, and reversible metabolically-stable cAMP antagonist that inhibits cAMP-dependent protein kinase and shows preference for PKA type I.

• Synonym(s): Adenosine 3′,5′-cyclic Monophosphorothioate, 8-Bromo-, Rp-Isomer, Sodium Salt, Rp-8-Br-cAMPS, Na, PKA Inhibitor VI
• Empirical Formula (Hill Notation): C₁₀H₁₀BrN₅O₅PS · Na
• Molecular Weight: 446.15
• UNSPSC Code: 41106305
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥99% (HPLC)
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic)
• solubility: water: 40 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Description

General description

A potent, cell-permeable, and reversible metabolically-stable cAMP antagonist that inhibits cAMP-dependent protein kinase and shows preference for PKA type I. More lipophilic than cAMP antagonist Rp-cAMPS (Cat. No. 116814).

Cell-permeable, potent, reversible, and metabolically stable antagonist of cAMP that is resistant to the action of mammalian cyclic nucleotide phosphodiesterases. Rp-8-Br-cAMPS is more lipophilic as compared to 8-Br-cAMP and Rp-cAMPS. In contrast to other common ATP-site inhibitors or to its parent compound Rp-cAMPS, Rp-8-Br-cAMPS discriminates between both isozymes of protein kinase A, preferring type I, thus providing additional selectivity. By occupying cAMP binding sites, prevents dissociation and activation of the kinase holoenzyme. λ = 264 nm (H₂O, pH 7.0); ε_max 17,000 M^-1cm^-1.

Note: 5 µmol = 2.23 mg.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
PKA 1

Product does not compete with ATP.

Reversible: yes

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Calaghan, S.C., et al. 1999. Pflugers Arch. 437, 780.
Gjertsen, B.T., et al. 1995. J. Biol. Chem. 270, 20599.
Yokozaki, H., et al. 1992. Cancer Res.52, 2504.
Dostmann, W.R. et al. 1990. J. Biol. Chem.265, 10484.

Legal Information

Sold under license of Patent DE 3,802,865.4 issued to BIOLOG LSI.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable