1.00950
Chromocult® Enterococci-Agar
Selective culture medium for the isolation and differentiation of Enterococci
Synonym(s):
Enterococcus Agar, Chromogenic Enterococci agar
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About This Item
UNSPSC Code:
41106213
NACRES:
NA.74
Quality Level
form
medium granules (dehydrated (DCM))
manufacturer/tradename
GranuCult®
technique(s)
microbiological culture: suitable
pH
6.8-7.2 (25 °C, 33 g/L in H2O)
solubility
33 g/L
application(s)
food and beverages
microbiology
storage temp.
15-25°C
suitability
Enterococcus spp.
Related Categories
General description
The presence of Enterococci, especially E. faecalis, E. faecium, E. durans and E. hirae, serves as an indicator for fecal contamination. The Enterococci-Agar medium is provided with a unique chromogenic substrate that is acted on by various Enterococci species to produce red colonies that can easily be identified. Non-Enterococci species produce blue/violet, turquoise or even colorless colonies which can easily be distinguished from the red. The medium is also provided with sodium azide and ox bile which inhibits the growth of any accompanying microbial flora.
Application
Chromocult® Enterococci-Agar can be used to isolate, differentiate and enumerate Enterococci present in various foodstuff, water or animal feed. It can also be used as for determination of fecal contamination in foodstuff, water or animal feed
Analysis Note
Appearance (clearness): clear
Appearance (color): yellowish
pH-value (25 °C): 6.8 - 7.2
Recovery on test medium (Enterococcus faecium ATCC 882): ≥ 50 %
Recovery on test medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 70 %
Recovery on test medium (Enterococcus durans ATCC 6056): ≥ 70 %
Recovery on test medium (Enterococcus hirae ATCC 8043 (WDCM 00089)): ≥ 70 %
Recovery on test medium (Bacillus cereus ATCC 11778 (WDCM 00001)): ≤ 0.01 %
Recovery on test medium (Escherichia coli ATCC 11775 (WDCM 00090)): ≤ 0.01 %
Recovery on test medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): ≤ 0.01 %
Colony color (Enterococcus faecium ATCC 882): red
Colony color (Enterococcus faecalis ATCC 19433 (WDCM 00009)): red
Colony color (Enterococcus durans ATCC 6056): red
Colony color (Enterococcus hirae ATCC 8043 (WDCM 00089)): red
Colony color (Aerococcus viridans ATCC 10400): pink-red to violet
Growth (Aerococcus viridans ATCC 10400): inhibited
Incubation: 24 hrs.; 35 °C; aerobic.
Appearance (color): yellowish
pH-value (25 °C): 6.8 - 7.2
Recovery on test medium (Enterococcus faecium ATCC 882): ≥ 50 %
Recovery on test medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 70 %
Recovery on test medium (Enterococcus durans ATCC 6056): ≥ 70 %
Recovery on test medium (Enterococcus hirae ATCC 8043 (WDCM 00089)): ≥ 70 %
Recovery on test medium (Bacillus cereus ATCC 11778 (WDCM 00001)): ≤ 0.01 %
Recovery on test medium (Escherichia coli ATCC 11775 (WDCM 00090)): ≤ 0.01 %
Recovery on test medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): ≤ 0.01 %
Colony color (Enterococcus faecium ATCC 882): red
Colony color (Enterococcus faecalis ATCC 19433 (WDCM 00009)): red
Colony color (Enterococcus durans ATCC 6056): red
Colony color (Enterococcus hirae ATCC 8043 (WDCM 00089)): red
Colony color (Aerococcus viridans ATCC 10400): pink-red to violet
Growth (Aerococcus viridans ATCC 10400): inhibited
Incubation: 24 hrs.; 35 °C; aerobic.
Legal Information
CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Certificates of Analysis (COA)
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