Skip to Content
Merck
All Photos(1)

Documents

932612

Sigma-Aldrich

NanoFabTx™ Biotin Lipid Mix

for synthesis of biotin-functionalized liposomes for small molecule delivery

Synonym(s):

Biotin-PEG-DSPE, Cholesterol, HSPC, PEG-DSPE

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352211
NACRES:
NA.25

Quality Level

100

storage temp.

−20°C

Application

NanoFabTx™ Biotin Lipid Mix is a ready-to-use nanoformulation lipid mixture for the synthesis of biotin-functionalized liposomes. This lipid mix enables users to encapsulate a wide variety of small molecules for targeting and immunotherapy applications without the need for lengthy trial-and-error optimization.

This product includes a curated lyophilized lipid mixture containing rationally selected lipids in precise ratios that have been optimized to achieve a desired size range of liposomes. Step-by-step protocols for the synthesis of liposomes surface-functionalized with biotin groups using extrusion and microfluidics are also included. The synthesized biotin-functionalized liposomes enable 1) selectively targeting cells which overexpress avidin-receptors (i.e. HeLa cells) and 2) the conjugation of avidin-tagged antibodies or proteins (i.e. Streptavidin) to the liposomes surface for immunotherapy applications and multi-targeting or multi-type cargo delivery.

Features and Benefits

  • A ready-to-use nanoformulation lipid blend for the synthesis of biotin-functionalized liposomes
  • Step-by-step extrusion and microfluidics protocols for liposome synthesis developed and tested by our formulation scientists
  • Flexible synthesis methods to create uniform and reproducible liposomes
  • Protocols and lipid blend optimized to make liposomes around 100 nm diameter with low polydispersity
  • Lipid mix optimized for avidin-tagged protein or antibody conjugation and small molecule encapsulation
Comprehensive protocols for liposome synthesis are included:
  • A lipid film hydration and extrusion protocol
  • A microfluidics protocol using commercial platforms or syringe pumps
The microfluidics protocol included with this product uses the NanoFabTx™ device kit (911593). These kits come with the microfluidics chips, fittings, and tubing required to get started with microfluidics-based synthesis (compatible microfluidics system or syringe pump required).

For more information, please refer to the protocol under the Protocol section of this page.

Legal Information

NANOFABTX is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Eliana B Souto et al.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 128, 27-35 (2018-11-26)
CAB51, a compact antibody against human epithelial growth receptor 2 (HER2, ErbB2), has been linked to cationic Solid Lipid Nanoparticles (SLN) via streptavidin-biotin interaction and their targeting potential evaluated against breast cancer cells. The amount of streptavidin and biotinylated antibody
Nina Pedersen et al.
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 62(2), 155-162 (2005-11-18)
Cationic solid lipid nanoparticles (SLN) have recently been suggested for non-viral gene delivery, as these particles consist of well tolerated substances, can bind DNA directly via electrostatic interactions and mediate gene transfer in vitro. We here report the development of

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service