- Exopolysaccharide Quantification for the Plant Pathogen Ralstonia solanacearum.
Exopolysaccharide Quantification for the Plant Pathogen Ralstonia solanacearum.
Bio-protocol (2017-05-20)
Rémi Peyraud, Timothy P Denny, Stéphane Genin
PMID34541063
RESUMEN
Soluble exopolysaccharide is a major virulence factor produced by the plant pathogen Ralstonia solanacearum. Its massive production during plant infection is associated with the arrest of water flow in xylem vessels leading eventually to plant death. The composition of this heavy macromolecule includes mainly N-acetylgalactosamine. Here we describe a colorimetric method for quantitative determination of the soluble exopolysaccharide present in culture supernatant of R. solanacearum.
MATERIALES
Referencia del producto
Marca
Descripción del producto
Sigma-Aldrich
Fosfato de potasio monobasic, powder, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%