66825-U
BIOshell A400 Protein C4 (3.4 μm) HPLC Columns
L × I.D. 10 cm × 2.1 mm, HPLC Column
Sinónimos:
Protein C4 Reversed-Phase Chromatography Column
About This Item
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Nombre del producto
BIOshell A400 Protein C4, 3.4 μm HPLC Column, 3.4 μm particle size, L × I.D. 10 cm × 2.1 mm
material
stainless steel hardware
Quality Level
agency
suitable for USP L26
description
Shell thickness (0.2 μm)
Solid Core (3.0 μm)
product line
BIOshell
feature
endcapped
manufacturer/tradename
BIOshell
packaging
1 ea of
extent of labeling
0.4% carbon loading
parameter
600 bar max. pressure
90 °C max. temp.
technique(s)
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
L × I.D.
10 cm × 2.1 mm
surface area
15 m2/g
surface coverage
4.2 μmol/m2
matrix
spherical silica particle platform
superficially porous particle
matrix active group
C4 (butyl) phase
particle size
3.4 μm
pore size
400 Å
operating pH range
2-9
separation technique
reversed phase
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Artículos
The article describes the use of difluoroacetic acid (DFA) as an effective alternative to acid modifiers, formic acid (FA) and trifluoroacetic acid (TFA) in the LC-UV/MS analysis of proteins.
-casein basis (electrophoresis), lyophilized powder; β-Casein from bovine milk, BioUltra, ≥98% (PAGE)
BIOshell™ A400 Protein C4 columns contain 3.4 μm particles with 400 Å pores, which are derivatized with dimethylbutyl silane and exhaustively endcapped for optimum protein recovery.
For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein
Protocolos
Larger porous shell particles with narrow particle size distribution, used in Supelco's BIOshell™ columns for the reversed-phase U/HPLC analysis of peptides and proteins, provide increased efficiency per unit pressure drop.
Optimized USP method for filgrastim, a recombinant form of human granulocyte colony stimulating factor (GCSF). Used in different biologics, it is crucial to know the purity of filgrastim.
HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell™ A400 Protein C4
Contenido relacionado
HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.
Global Trade Item Number
Número de referencia del producto (SKU) | GTIN |
---|---|
66825-U | 4061833388143 |
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