50758-U
Ascentis® Express RP-Amide, 5 μm HPLC Column
5 μm particle size, L × I.D. 15 cm × 3 mm
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About This Item
UNSPSC Code:
41115700
NACRES:
SB.52
material
stainless steel column
Quality Level
agency
suitable for USP L60
product line
Ascentis®
feature
endcapped
manufacturer/tradename
Ascentis®
packaging
1 ea of
parameter
60 °C temp. range
600 bar max. pressure (9000 psi)
technique(s)
HPLC: suitable
LC/MS: suitable
L × I.D.
15 cm × 3 mm
surface area
90 m2/g
impurities
<5 ppm metals
matrix
Fused-Core® particle platform
superficially porous particle
matrix active group
amide, alkyl phase
particle size
5 μm
pore size
90 Å pore size
pH range
2-9
application(s)
food and beverages
separation technique
reversed phase
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General description
Ascentis Express 5 μm RP-Amide is a high-speed, high-performance liquid chromatography column based on the highly efficient Fused-Core® particle design. The Fused-Core particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.6 μm thick porous shell and the highly uniform overall particle size of 5 μm. The one-step bonded, endcapped, amide-based, polar-embedded stationary phase of Ascentis Express 5 μm RP-Amide provides a stable, reversed-phase packing with decreased hydrophobic character. Ascentis Express RP-Amide can be used for basic, acidic, or neutral compounds with orthogonal selectivity from C18 or C8. The polar-embedded Ascentis Express 5 μm RP-Amide columns are compatible with 100% aqueous mobile phases without suffering from “phase collapse”.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
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Today sub-2 microm packed columns are very popular to conduct fast chromatographic separations. The mass-transfer resistance depends on the particle size but some practical limits exist not to reach the theoretically expected plate height and mass-transfer resistance. Another approach applies
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