ZRB2839

Anti-CD5 Antibody, clone 1M19 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinónimos: LEU1, Lymphocyte antigen T1/Leu-1
• UNSPSC Code: 12352203

Propiedades

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1M19, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 54.58 kDa; observed mol wt ~70 kDa
• purified by: using Protein A
• species reactivity: human
• species reactivity (predicted by homology): monkey
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgGκ
• epitope sequence: Extracellular domain
• Protein ID accession no.: NP_055022
• UniProt accession no.: P06127
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... CD5(921)

Descripción

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1M19 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects CD5. It targets an epitope within 22 amino acids from the extracellular domain.

Immunogen

KLH-conjugated linear peptide corresponding to 22 amino acids from the extracellular domain of human CD5.

Application

Quality Control Testing

Evaluated by Western Blotting in human liver tissue lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected CD5 in human liver tissue lysate.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CD5 in human spleen tissue sections and in human tonsil tissue sections.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected CD5 in MOLT-4 cells.

Flow Cytometry Analysis: 1 µg from a representative lot detected CD5 in one million human peripheral blood mononuclear cells (PBMC).

Affinity Binding Assay: A representative lot of this antibody bound CD5 peptide with at least ten thousand-fold (10,000X) higher than with non-specific control peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

T-cell surface glycoprotein CD5 (UniProt: P06127; also known as Lymphocyte antigen T1/Leu-1) is encoded by the CD5 (also known as LEU1) gene (Gene ID: 921) in human. CD5 is a single-pass type I membrane glycoprotein that belongs to the scavenger receptor cysteine-rich superfamily. It features a large extracellular domain composed of three scavenger receptor cysteine-rich (SRCR) domains, a transmembrane region, and a cytoplasmic tail. CD5 plays a critical role in modulating T and B cell responses. It acts primarily as an inhibitor of T-cell receptor (TCR) and B-cell receptor (BCR) signaling. CD5 can modulate the threshold for T-cell activation and differentiation, thus influencing the immune response′s strength and quality. It also affects the survival and differentiation of a specific subset of B cells known as B-1 cells. CD5 is predominantly expressed on T cells and a subset of B cells (B-1 cells) in humans. The presence of CD5 on B cells is associated with the production of natural antibodies and autoantibodies. CD5 expression and function are associated with various autoimmune diseases and lymphoid malignancies. Overexpression of CD5 has been observed in certain types of leukemia and lymphomas, such as chronic lymphocytic leukemia and mantle cell lymphoma. In autoimmune diseases, aberrant CD5 signaling or expression can contribute to the pathology of conditions like systemic lupus erythematosus and rheumatoid arthritis, by influencing the activation and survival of autoreactive B and T cells.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable