ZRB2761

Anti-Mitofusin-1 Antibody, clone 1I7 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinónimos: Fzo homolog, Transmembrane GTPase MFN1
• UNSPSC Code: 12352203
• NACRES: NA.43

Propiedades

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: recombinant monoclonal
• description: clone 1I7 ; recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 84.16 kDa; observed mol wt ~84 kDa
• purified by: using Protein A
• species reactivity: human
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): affinity binding assay: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal
• Protein ID accession no.: NP_284941.2
• UniProt accession no.: Q8IWA4
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... MFN1(55669)

Descripción

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1I7 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Mitofusin-1. It targets an epitope within 24 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal region of human Mitofusin-1.

Application

Quality Control Testing

Evaluated by Western Blotting in PC3 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Mitofusin-1 in PC3 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Mitofusin-1 in K562 cell lysate.

Affinity Binding Assay: A representative lot of this antibody bound Mitofusin-1 peptide with a KD of 2.4 x 10-8 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Mitofusin-1 in Human kidney tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Mitofusin-1 (UniProt: Q8IWA4; also known as Fzo homolog, Transmembrane GTPase MFN1) is encoded by the MFN1 gene (Gene ID: 55669) in human. Mitofusin-1 and -2 (Mfn1 and Mfn2) are dynamin-related GTPase homologous proteins that are localized at the outer mitochondrial membrane and are essential for mitochondria fusion. They are thought to fuse adjacent mitochondria via concerted oligomerization and GTP hydrolysis. They also play a role in shaping the endoplasmic reticulum-mitochondria interface. They share about 80% sequence homology and contain homologous functional domains. Mitofusin-1 is a nuclear coded, homodimeric protein that can also form homomultimers and heteromultimers with mitofusin -2. It is ubiquitously distributed, but higher expression is observed in kidney and heart tissue. Mfn1 contains two cytoplasmic domains (aa 1-584; 630-741), two transmembrane domains (aa 585-605; 609-629) and a short mitochondrial intermembrane domain (aa 606-608). Its GTPase region is composed of a central β sheet with three helices on each side. Silencing of MFN1 gene is reported to inhibit mitochondrial fusion and subsequently reduced ATP production. Three isoforms of Mfn1 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Cao, Y-L., et al. (2017). Nature. 542(7641); 372-376; Qi, Y., et al. (2016). J. Cell Biol. 215(5); 621-629; Ishihara, N., et al. (2004). J. Cell Sci. 117(26); 6535-6546).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable