Saltar al contenido
Merck

Y4770

Sigma-Aldrich

Anti-YAP1 (C-terminal) antibody produced in rabbit

enhanced validation

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Sinónimos:

Anti-YAP, Anti-YAP2, Anti-YAP65, Anti-Yes-associated protein 1 65 kDa

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.44

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~65 kDa

species reactivity

human

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1 mg/mL

technique(s)

western blot: 0.5-1 μg/mL using HEK-293T cells co-transfected with human YAP1 and c-Abl

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... YAP1(10413)
mouse ... Yap1(22601)
rat ... Yap1(363014)

General description

YAP1 (Yes-associated protein 1, YAP, YAP2, YAP65) is a modular adaptor protein with multiple protein interaction domains, that is based on its interaction with Src-family tyrosine kinase c-Yes. It has a SH3-binding motif, proline-rich amino terminus, a WW domain, a coiled-coil and a PDZ-binding motif at the extreme C-terminus. YAP1 also contains a 14-3-3 interacting motif.

Specificity

Anti-YAP1 (C-terminal) antibody is specific for human YAP1. The product is expected to react with mouse, bovine, and dog YAP1. In immunoblotting, detection of the YAP1 band is specifically inhibited with the immunizing peptide.

Immunogen

synthetic peptide corresponding to amino acids 442-454 located at the C-terminus of human YAP1. The sequence is identical in mouse, bovine, and dog YAP1.

Application

Anti-YAP1 (C-terminal) antibody is suitable for use in western blot (.5-1 μg/mL) using HEK-293T cells co-transfected with human YAP1 and c-Abl.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
Western Blotting (1 paper)

Biochem/physiol Actions

YAP1 (Yes-associated protein 1) induces p73-dependent apoptosis through the specific and selective co-activation of p53AIP1, an apoptotic p73 target gene. It stabilizes p73 by preventing Itch-mediated ubiquitination of p73. Upon phosphorylation by Akt at Ser127, 14-3-3 is recruited and stimulates YAP1 localization to the cytoplasm, resulting in loss of co-activator function in the nucleus. Inhibition of Akt potentiates the nuclear re-localization of YAP1 to promote apoptosis by p73.
YAP1 is a WW domain containing protein that functions as a transcriptional co-activator . It may also regulate p73 gene function in response to DNA damage. YAP1 is known to associate with a wide range of transcription factors . Alterations in YAP1 have been associated with tumor development .

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

¿No encuentra el producto adecuado?  

Pruebe nuestro Herramienta de selección de productos.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

The transcriptional coactivator Yes-associated protein drives p73 gene-target specificity in response to DNA Damage
Strano S, et al.
Molecular Cell, 18(4), 447-459 (2005)
Akt phosphorylates the Yes-associated protein, YAP, to induce interaction with 14-3-3 and attenuation of p73-mediated apoptosis
Basu S, et al.
Molecular Cell, 11(1), 11-23 (2003)
The Yes-associated protein 1 stabilizes p73 by preventing Itch-mediated ubiquitination of p73
Levy D, et al.
Cell Death and Differentiation, 14(4), 743-743 (2007)
Alexander von Gise et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(7), 2394-2399 (2012-02-07)
Heart growth is tightly controlled so that the heart reaches a predetermined size. Fetal heart growth occurs through cardiomyocyte proliferation, whereas postnatal heart growth involves primarily physiological cardiomyocyte hypertrophy. The Hippo kinase cascade is an important regulator of organ growth.
Pingping Zhu et al.
Nature communications, 7, 13608-13608 (2016-12-03)
Liver cancer stem cells (CSCs) may contribute to the high rate of recurrence and heterogeneity of hepatocellular carcinoma (HCC). However, the biology of hepatic CSCs remains largely undefined. Through analysis of transcriptome microarray data, we identify a long noncoding RNA

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico