Y2627

2xYT Medium

EZMix Powder microbial growth medium

• UNSPSC Code: 41106200
• NACRES: NA.85

Propiedades

• grade: for molecular biology
• description: nutrient rich, quick dissolve, dust free formulation
• sterility: non-sterile
• form: powder
• composition: Inert binder (EZMix only), 0.6 g/L ; NaCl, 5 g/L ; Tryptone (pancreatic digest of casein), 16 g/L ; Yeast extract, 10 g/L
• application(s): agriculture; food and beverages
• storage temp.: room temp
• suitability: nonselective for Escherichia coli; nonselective for coliforms

Descripción

General description

2xYT is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Application

Suitable for non-selective cultivation of M13 bacteriophage, and E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

Features and Benefits

2xYT EZMix powder provides:

• Granulated, dust-free format for safer handling and faster mixing
• Easy scale-up using larger package size
• Standard formulation

Reconstitution

Stir to suspend 31.6 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Legal Information

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)