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Merck

Y2021

Sigma-Aldrich

Yeast Synthetic Drop-out Medium Supplements

without histidine, leucine, tryptophan, and adenine

Sinónimos:

Yeast Synthetic Drop-out Media Supplements

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About This Item

MDL number:
UNSPSC Code:
41106212
NACRES:
NA.85

Quality Level

form

powder

technique(s)

transformation: suitable

application(s)

microbiology

storage temp.

room temp

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Application

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.
Yeast Synthetic Drop-out Medium Supplements, without histidine, leucine, tryptophan, and adenine has been used in yeast two-hybrid screening.

Other Notes

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Preparation Note

Dissolve 1.39 g of supplement per liter of medium.

pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Artículos

Technical Article on yeast media. Yeasts are eukaryotic microorganisms whose genomes have been comprehensively studied and some have been sequenced.

Protocolos

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.

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