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Merck

XNAP2

Sigma-Aldrich

Extract-N-Amp Plant PCR Kit

sufficient for 100 extractions, sufficient for 100 amplifications

Sinónimos:

Plant direct PCR kit

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About This Item

UNSPSC Code:
41106303
NACRES:
NA.55

usage

sufficient for 100 amplifications
sufficient for 100 extractions
sufficient for 100 reactions

Quality Level

feature

dNTPs included
hotstart

technique(s)

PCR: suitable

color

colorless

application(s)

agriculture

shipped in

wet ice

storage temp.

−20°C

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General description

The Extract-N-Amp Plant PCR Kits contain all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. A novel Extraction Solution eliminates the need for conventional freezing of plant tissues with liquid nitrogen, mechanical disruption, organic extraction, column purification, or precipitation of DNA. The kit also includes a PCR ReadyMix, especially formulated for amplification directly from extract.

Genomic DNA is extracted from 0.5 to 0.7 cm plant leaf disks that have been cut with a standard paper punch and simply incubated in Extraction Solution at 95 oC for 10 minutes. An equal volume of Dilution Solution is added to the extract to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and the Extract-N-Amp PCR ReadyMix, included in the kit.
The Extract-N-Amp Plant PCR Kits are intended for direct PCR (polymerase chain reaction). This kit contains all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. It is a novel Extraction Solution that eliminates the need for conventional freezing of plant tissues.

Application

Extract-N-Amp Plant PCR Kit has been used for genomic DNA isolation from plants and the fungal mycelium. It has also been used in polymerase chain reaction (PCR).
Extract-N-Amp Plant PCR Kit is suitable for use in genotyping.

It has been used forDNA extraction from fresh mushrooms.

Features and Benefits

  • Single-step extraction of plant genomic DNA for PCR in less than 15 minutes
  • A PCR ReadyMix, specially formulated for amplification directly from extract
  • Hot Start antibody for highly specific PCR amplification of genomic DNA
  • Extract stable at 4 °C for at least 6 months
  • Plant genomic DNA can be extracted for PCR in under 15 minutes using a method.
  • No freezing, mechanical disruption, organic extraction, column purification or precipitation is necessary
  • A specially formulated PCR ReadyMix is provided for use with the extracted DNA
  • The Hot Start antibody ensures highly specific PCR amplification of genomic DNA
  • Suitable for high-throughput plant genetic analysis.
  • Lengthy enzymatic digestions are not required, saving time and effort.
  • The extracted DNA remains stable at 4 °C for atleast 6 months.

Components

Extract-N-Amp Plant PCR Kits contain Dilution solution, Extraction solution, and Extract-N-Amp PCR Reaction Mix (2X) (buffer, salts, dNTPs, Taq polymerase, and JumpStart Taq antibody)

Other Notes

For additional information, please see www.sigma-aldrich.com/extract-n-amp.
The Extract-N-Amp Plant PCR Kits are for laboratory use only. Not for drug, household, or other uses.

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

related product

Referencia del producto
Descripción
Precios

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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Elin Videvall et al.
mSystems, 2(6) (2017-11-29)
The gut microbiome of animals is emerging as an important factor influencing ecological and evolutionary processes. A major bottleneck in obtaining microbiome data from large numbers of samples is the time-consuming laboratory procedures required, specifically the isolation of DNA and
Ante Karoglan et al.
Acta dermato-venereologica, 99(13), 1253-1257 (2019-10-02)
Imbalance in skin microflora, particularly related to certain Cutibacterium acnes strains, may trigger acne. Application of non-acne-causing strains to the skin may modulate the skin microbiome and thereby lead to a reduction in acne. This pilot study evaluates the safety
Phylogenetic placements and cultural characteristics of Tuber species isolated from ectomycorrhizas
Obase K, et al.
Mycoscience (2021)
Dionne N Shepherd et al.
Journal of virological methods, 149(1), 97-102 (2008-02-19)
A high-throughput method of isolating and cloning geminivirus genomes from dried plant material, by combining an Extract-n-Amp-based DNA isolation technique with rolling circle amplification (RCA) of viral DNA, is presented. Using this method an attempt was made to isolate and
José Dijair Antonino de Souza Júnior et al.
PloS one, 8(12), e85364-e85364 (2014-01-07)
The root-knot nematode Meloidogyne incognita causes serious damage and yield losses in numerous important crops worldwide. Analysis of the M. incognita genome revealed a vast number of proteases belonging to five different catalytic classes. Several reports indicate that M. incognita

Artículos

The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that are available and help you find the best kit suited for your needs.

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

Protocolos

Extract-N-Amp™ Plant PCR Kits Protocol

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