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T8768

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate

horseradish peroxidase substrate, chromogenic, ≥98%, powder

Sinónimos:

TMB dihydrochloride

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About This Item

Fórmula lineal:
[-C6H2(CH3)2-4-NH2]2·2HCl·xH2O
Número de CAS:
207738-08-7
Peso molecular:
313.27 (anhydrous basis)
MDL number:
MFCD00150104
UNSPSC Code:
12352204
PubChem Substance ID:
24900558
NACRES:
NA.83

product name

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate, powder, ≥98%

Quality Level

200

assay

≥98%

form

powder

solubility

acetic acid: water (1:2): 20 mg/mL, clear to slightly hazy

storage temp.

2-8°C

SMILES string

O.Cl.Cl.Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2.2ClH.H2O/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;;/h5-8H,17-18H2,1-4H3;2*1H;1H2

InChI key

KVCWTKDFVVSVSJ-UHFFFAOYSA-N

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General description

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate (TMB-d) is a sensitive chromogen substrate for peroxidase. It is non- carcinogenic, hence, it can be used as a safe substitute for benzidine. TMB-d is useful in quantifying hemoglobin and in staining myeloperoxidase-containing granules in granulocytes.

Application

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate has also been used as a horseradish peroxidase substrate in enzyme linked immunosorbent assay and reverse dot blot (RDB).
Noncarcinogenic substitute for benzidine.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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