T5941
Clorhidrato de Trizma®
BioPerformance Certified, suitable for cell culture, ≥99.0% (titration)
Sinónimos:
Clorhidrato de trometano, TRIS / HCI, TRIS hydrochloride, Tris(hidroximetil)-aminometano hydrochloride
About This Item
Productos recomendados
Quality Level
grade
BioPerformance Certified
assay
≥99.0% (titration)
form
crystalline powder
technique(s)
DNA purification: suitable
cell culture | mammalian: suitable
electrophoresis: suitable
protein extraction: suitable
impurities
DNAse, Exonuclease; NICKase, Endonuclease; RNAse and Protease, none detected
endotoxin and total aerobic microbial count, tested
≤0.5% water (Karl Fischer)
color
white
useful pH range
7.0-9.0
pKa (25 °C)
8.1
mp
150-152 °C
solubility
H2O: 667 mg/mL
density
1.39 g/cm3 at 20—25 °C
1.40 g/cm3 at 20—25 °C
absorption
≤0.05 at 290 at 40%
suitability
suitable for electrophoresis
suitable for immunohistochemistry
suitable for molecular biology
application(s)
diagnostic assay manufacturing
general analytical
life science and biopharma
sample preparation
SMILES string
Cl.NC(CO)(CO)CO
InChI
1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
InChI key
QKNYBSVHEMOAJP-UHFFFAOYSA-N
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General description
Tris buffers are indispensable in techniques like protein electrophoresis and western blotting. In many western blot protocols, a low ionic strength Tris buffer is employed for protein transfer. The transfer duration depends on the blotting apparatus and the size range of peptides under investigation. Tris-based buffers are also commonly used in SDS-PAGE gels, running buffers, and blotting buffers.
Furthermore, Tris salts find extensive use in DNA agarose electrophoresis, particularly in the preparation of buffers like TAE (Tris acetate/EDTA) and TBE (Tris borate/EDTA). These buffers play a pivotal role in various applications, including biochemistry, protein purification, and electrophoresis. Tris HCl is a versatile and essential for maintaining the pH of biological systems and ensuring the optimal performance of biochemical, cell culture, and molecular biology techniques.
Application
- as a washing and rinsing buffer in an immunohistochemical analysis(1)
- as a component of reducing sample buffer, lysis buffer and elution buffer, in a biochemical research involving gel electrophoresis and BioLayer interferometry measurement(2)
Features and Benefits
- Suitable for Molecular Biology and Cell Culture
- Can be used as a Buffer component, for Electrophoresis and Protein separation
- Tested for Endotoxins and Total Aerobic Microbial Count
- Free from DNase, NICKase, RNase, and Protease
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
- Effective Buffering from pH 7-9 (25 °C) with a pKa of 8.1 (25 °C)
- Highly soluble in water
Packaging
Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.
Other Notes
Legal Information
comparable product
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Protocolos
election of Poly(A)+ RNA by Oligo(dT)-Cellulose Chromatography
Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.
In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry
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