SRP6372

Cytidine deaminase human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Sinónimos: CDA, CDD, Cytidine aminohydrolase, Cytosine nucleoside deaminase
• UNSPSC Code: 12352200
• NACRES: NA.32

Propiedades

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥90% (SDS-PAGE)
• form: liquid
• mol wt: 18.3 kDa (166 aa, 1-146 aa + His Tag.)
• packaging: pkg of 100 μg
• concentration: 0.5 mg/mL
• UniProt accession no.: P32320
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... CDA(978)

Descripción

General description

Cytidine deaminase (CDA) is an enzyme that scavenges exogenous and endogenous cytidine and 2′-deoxycytidine for UMP synthesis. This protein is one of several deaminases responsible for maintaining the cellular pyrimidine pool. CDA also catalyzes the deamination of chemotherapeutic cytosine nucleoside analogs such as arabinofuranosyl cytidine (Ara-C) and 5-azacytidine, which results in the loss of their cytotoxic and antitumor function.

Biochem/physiol Actions

Cytidine deaminase (CDA) is mainly expressed in granulocytes and can form homotetramers. Recombinant human CDA protein, fused to His-tag at N-terminus, was expressed in Escherichia coli and purified by using conventional chromatography.

Physical form

0.5 mg/mL in 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 1 mM DTT, 2 mM EDTA and 40% glycerol.

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable