SRP0713

PFTK1 (CDK14)/CYCLINY, Active

PRECISIO^® Kinase, recombinant

• UNSPSC Code: 12352202
• NACRES: NA.77

Propiedades

• recombinant: expressed in baculovirus infected Sf9 cells
• assay: 70-95% (SDS-PAGE)
• form: frozen liquid
• specific activity: 2.0-3.5 nmol/min·mg (radiometric)
• mol wt: ~64 kDa (Cyclin Y); ~72 kDa (PFTK1 (CDK14))
• concentration: 0.1 μg/μL
• accession no.: NM_012395; NM_145012
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... CCNY(219771) , CDK14(5218)

Descripción

Application

Useful for Kinase assay and Western blot.

Biochem/physiol Actions

Recombinant human full-length PFTK1 (CDK14) and Cyclin Y (2-end) were co-expressed by Baculovirus in Sf9 insect cells using N-terminal GST tags. PFTK1 is a member of the CDC2 - related protein kinase family which is expressed primarily in the postnatal and adult nervous system ⁽¹⁾. PFTK1 is expressed at high levels in brain, pancreas, kidney, heart, testis, and ovary. PFTK1 amino acid residue ser119 is required for its interaction with all four 14-3-3 isoforms, while ser120 may contribute slightly to PFTK1 binding ⁽²⁾. PFTK1 acts as a cyclin-dependent kinase that regulates cell cycle progression and cell proliferation. Overexpression of PFTK1 predicts resistance to chemotherapy in patients with oesophageal squamous cell carcinoma.

Reconstitution

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Storage and Stability

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable