SRP0286

Sphingosine kinase 2 Active from mouse

recombinant, expressed in baculovirus, ≥70% (SDS-PAGE)

• Sinónimos: SK2, SPHK2, SPK 2
• UNSPSC Code: 12352204
• NACRES: NA.54

Propiedades

• recombinant: expressed in baculovirus
• assay: ≥70% (SDS-PAGE)
• form: aqueous solution
• specific activity: ≥2 units/μg
• mol wt: 68 kDa
• NCBI accession no.: NM_203280
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: mouse ... SPHK2(56848)

Descripción

General description

Mouse Sphingosine kinase 2 ( GenBank Accession No. NM_203280), full length with N-terminal His tag, MW = 68 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Sphingosine kinase 2 is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Sphingosine kinase 2 has been used in a study to assess blood–brain barrier protection in hypoxic preconditioning-induced cerebral ischemic tolerance. Sphingosine kinase 2 has also been used in a study to investigate sensitization of human colon cancer cells to sodium butyrate-induced apoptosis.

Unit Definition

One unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.