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Documentos clave

SRP0107

Sigma-Aldrich

HDAC-5 full length human

recombinant, expressed in baculovirus infected insect cells, ≥10% (SDS-PAGE)

Sinónimos:

HD5, Histone deacetylase 5, NY-CO-9

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected insect cells

assay

≥10% (SDS-PAGE)

form

aqueous solution

potency

≥1 units/mg

mol wt

150 kDa

packaging

pkg of 5 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

NM_001015053

UniProt accession no.

Q9UQL6

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... HDAC5(10014)

General description

Histone deacetylase 5 (HDAC5) belongs to the Hda1-like proteins class IIa HDAC family. It contains 17 phospho-acceptor residues and a classical deacetylase domain. The HDAC5 gene is mapped to human chromosome 17q21.

Application

HDAC-5 full-length human has been used in the adenosine diphosphate (ADP)-Glo assay and Γ-p32-adenosine triphosphate (ATP) radioisotope assay with focal adhesion kinase (FAK) kinase.

Biochem/physiol Actions

Histone deacetylase 5 (HDAC5) mediates synoviocyte activation, neural repair and regeneration. It regulates the assembly of the pericentric heterochromatin. Elevated expression of HDAC5 is implicated in high-risk medulloblastoma and breast cancer in very young women (BCVY).

Unit Definition

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

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Certificados de análisis (COA)

Lot/Batch Number
50045100706
5004500706

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beta-Adrenergic Stimulation Induces Histone Deacetylase 5 (HDAC 5) Nuclear Accumulation in Cardiomyocytes by B55alpha-PP 2A-Mediated Dephosphorylation
Weeks KL, et al.
Journal of the American Heart Association null
Edward Seto et al.
Cold Spring Harbor perspectives in biology, 6(4), a018713-a018713 (2014-04-03)
Histone deacetylases (HDACs) are enzymes that catalyze the removal of acetyl functional groups from the lysine residues of both histone and nonhistone proteins. In humans, there are 18 HDAC enzymes that use either zinc- or NAD(+)-dependent mechanisms to deacetylate acetyl
Tadatoshi Sato et al.
Nature communications, 11(1), 3282-3282 (2020-07-03)
Osteocytes, cells ensconced within mineralized bone matrix, are the primary skeletal mechanosensors. Osteocytes sense mechanical cues by changes in fluid flow shear stress (FFSS) across their dendritic projections. Loading-induced reductions of osteocytic Sclerostin (encoded by Sost) expression stimulates new bone

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Huntington's disease (HD) is an autosomal dominant, late-onset neurodegenerative disorder characterized by a selective neuronal cell death in the cortex and striatum leading to cognitive dysfunction, motor impairment and behavioral changes.

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