SRE0001

Pepsina from porcine gastric mucosa

Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, ≥3200 units/mg protein

• Sinónimos: Pepsina A, Pepsina from hog stomach
• Número de CAS: 9001-75-6
• Comisión internacional de enzimas: 3.4.23.1 (BRENDA, IUBMB)
• EC Number: 232-629-3
• MDL number: MFCD00081840
• UNSPSC Code: 12352204
• NACRES: NA.54

Propiedades

• Quality Level: 400
• form: lyophilized powder
• specific activity: ≥3200 units/mg protein
• mol wt: 35 kDa
• impurities: salt, essentially free
• color: white to off-white
• UniProt accession no.: P00791
• application(s): diagnostic assay manufacturing
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: pig ... LOC396892(396892)

Descripción

Application

Puede utilizarse hidrólisis con pepsina para producir los fragmentos F(ab′)₂ de los anticuerpos. pepsina en www.sigma-aldrich.com/enzymeexplorer

Biochem/physiol Actions

Escisión preferente: residuos hidrófobos y aromáticos en las posiciones P1 y P1′. Rompe los enlaces Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe y Phe-Tyr en la cadena β de la insulina

Unit Definition

One unit will produce a ΔA₂₈₀ of 0.001 per min at pH2.0 at 37° C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16mL. Light path = 1cm.)

Analysis Note

pH óptimo es 2-4. Activa en urea 4 M y HCl de guanidina 3 M. Estable a 60 ºC. La pepsina se desactiva de manera irreversible a pH 8,0 - 8,5.

Protein determined by E1%/280

Other Notes

View more information on pepsin at www.sigma-aldrich.com/enzymeexplorer.

Información de seguridad

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable